Cell Reports Medicine (Sep 2020)

Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant

  • Petra Mlcochova,
  • Dami Collier,
  • Allyson Ritchie,
  • Sonny M. Assennato,
  • Myra Hosmillo,
  • Neha Goel,
  • Bo Meng,
  • Krishna Chatterjee,
  • Vivien Mendoza,
  • Nigel Temperton,
  • Leo Kiss,
  • Leo C. James,
  • Katarzyna A. Ciazynska,
  • Xiaoli Xiong,
  • John A.G. Briggs,
  • James A. Nathan,
  • Federica Mescia,
  • Laura Bergamaschi,
  • Hongyi Zhang,
  • Petros Barmpounakis,
  • Nikos Demeris,
  • Richard Skells,
  • Paul A. Lyons,
  • John Bradley,
  • Steven Baker,
  • Jean Pierre Allain,
  • Kenneth G.C. Smith,
  • Rachel Bousfield,
  • Michael Wilson,
  • Dominic Sparkes,
  • Glenn Amoroso,
  • Effrosyni Gkrania-Klotsas,
  • Susie Hardwick,
  • Adrian Boyle,
  • Ian Goodfellow,
  • Ravindra K. Gupta,
  • Stephen Baker,
  • John Bradley,
  • Gordon Dougan,
  • Ian Goodfellow,
  • Ravi Gupta,
  • Paul J. Lehner,
  • Paul Lyons,
  • Nicholas J. Matheson,
  • Kenneth G.C. Smith,
  • Mark Toshner,
  • Michael P. Weekes,
  • Nick Brown,
  • Martin Curran,
  • Surendra Palmar,
  • Hongyi Zhang,
  • David Enoch,
  • Daniel Chapman,
  • Ashley Shaw,
  • Sherly Jose,
  • Areti Bermperi,
  • Julie Ann Zerrudo,
  • Evgenia Kourampa,
  • Laura Watson,
  • Jieniean Worsley,
  • Caroline Saunders,
  • Ranalie de Jesus,
  • Jason Domingo,
  • Ciro Pasquale,
  • Bensi Vergese,
  • Phoebe Vargas,
  • Marivic Fabiculana,
  • Marlyn Perales,
  • Lee Mynott,
  • Elizabeth Blake,
  • Amy Bates,
  • Anne-Laure Vallier,
  • Alexandra Williams,
  • David Phillips,
  • Edmund Chiu,
  • Alex Overhill,
  • Nicola Ramenatte,
  • Jamal Sipple,
  • Steven Frost,
  • Helena Knock,
  • Richard Hardy,
  • Emily Foster,
  • Fiona Davidson,
  • Viona Rundell,
  • Purity Bundi,
  • Richmond Abeseabe,
  • Sarah Clark,
  • Isabel Vicente,
  • Laura Watson,
  • Jieniean Worsley,
  • Anne Elmer,
  • Carla Ribeiro,
  • Jenny Kourampa,
  • Sherly Jose,
  • Jane Kennet,
  • Jane Rowlands,
  • Anne Meadows,
  • Criona O’Brien,
  • Rebecca Rastall,
  • Cherry Crucusio,
  • Sarah Hewitt,
  • Jane Price,
  • Jo Calder,
  • Laura Canna,
  • Ashlea Bucke,
  • Hugo Tordesillas,
  • Julie Harris,
  • Valentina Ruffolo,
  • Jason Domingo,
  • Barbara Graves,
  • Helen Butcher,
  • Daniela Caputo,
  • Emma Le Gresley,
  • Benjamin J. Dunmore,
  • Jennifer Martin,
  • Ekaterina Legchenko,
  • Carmen Treacy,
  • Christopher Huang,
  • Jennifer Wood,
  • Rachel Sutcliffe,
  • Josh Hodgson,
  • Joy Shih,
  • Stefan Graf,
  • Zhen Tong,
  • Federica Mescia,
  • Tobias Tilly,
  • Ciara O’Donnell,
  • Kelvin Hunter,
  • Linda Pointon,
  • Nicole Pond,
  • Marta Wylot,
  • Emma Jones,
  • Stuart Fawke,
  • Ben Bullman,
  • Lori Turner,
  • Isobel Jarvis,
  • Ommar Omarjee,
  • Aloka De Sa,
  • Joe Marsden,
  • Ariana Betancourt,
  • Marianne Perera,
  • Maddie Epping,
  • Nathan Richoz,
  • Georgie Bower,
  • Rahul Sharma,
  • Francesca Nice,
  • Oisin Huhn,
  • Stuart Fawke,
  • Natalia Savoinykh Yarkoni,
  • Nika Romashova,
  • Daniel Lewis,
  • Andrew Hinch,
  • Chiara Cossetti,
  • Mateusz Strezlecki,
  • Richard Grenfell,
  • Hannah Stark,
  • Neil Walker,
  • Kathy Stirrups,
  • Nigel Ovington,
  • Eleanor Dewhust,
  • Emily Li,
  • Sofia Papadia,
  • Nathalie Kingston,
  • Andrew Lever,
  • Estee Torok,
  • William Hamilton,
  • Grant Hall,
  • Aminu Jahun,
  • Yasmin Chaudhry,
  • Malte Pinckert,
  • Iliana Georgana,
  • Anna Yakovleva,
  • Laura Caller,
  • Sarah Caddy,
  • Theresa Feltwell,
  • Fahad Khokhar,
  • Luke Meredith,
  • Charlotte Holdcroft,
  • Martin Curran,
  • Surendra Parmar,
  • Nathalie Kingston,
  • Andrew Lever,
  • Estee Torok,
  • William Hamilton,
  • Grant Hall,
  • Aminu Jahun,
  • Yasmin Chaudhry,
  • Malte Pinckert,
  • Iliana Georgana,
  • Anna Yakovleva,
  • Laura Caller,
  • Sarah Caddy,
  • Theresa Feltwell,
  • Fahad Khokhar,
  • Luke Meredith,
  • Charlotte Holdcroft,
  • Martin Curran,
  • Surendra Parmar

Journal volume & issue
Vol. 1, no. 6
p. 100099

Abstract

Read online

Summary: Rapid COVID-19 diagnosis in the hospital is essential, although this is complicated by 30%–50% of nose/throat swabs being negative by SARS-CoV-2 nucleic acid amplification testing (NAAT). Furthermore, the D614G spike mutant dominates the pandemic and it is unclear how serological tests designed to detect anti-spike antibodies perform against this variant. We assess the diagnostic accuracy of combined rapid antibody point of care (POC) and nucleic acid assays for suspected COVID-19 disease due to either wild-type or the D614G spike mutant SARS-CoV-2. The overall detection rate for COVID-19 is 79.2% (95% CI 57.8–92.9) by rapid NAAT alone. The combined point of care antibody test and rapid NAAT is not affected by D614G and results in very high sensitivity for COVID-19 diagnosis with very high specificity.

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