Proteomic Characterization of Bacteriophage Peptides from the Mastitis Producer <i>Staphylococcus aureus</i> by LC-ESI-MS/MS and the Bacteriophage Phylogenomic Analysis
Ana G. Abril,
Mónica Carrera,
Karola Böhme,
Jorge Barros-Velázquez,
Benito Cañas,
José-Luis R. Rama,
Tomás G. Villa,
Pilar Calo-Mata
Affiliations
Ana G. Abril
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, 15898 Santiago de Compostela, Spain
Mónica Carrera
Department of Food Technology, Spanish National Research Council, Marine Research Institute, 36208 Vigo, Spain
Karola Böhme
Agroalimentary Technological Center of Lugo, 27002 Lugo, Spain
Jorge Barros-Velázquez
Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, 27002 Lugo, Spain
Benito Cañas
Department of Analytical Chemistry, Complutense University of Madrid, 28040 Madrid, Spain
José-Luis R. Rama
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, 15898 Santiago de Compostela, Spain
Tomás G. Villa
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, 15898 Santiago de Compostela, Spain
Pilar Calo-Mata
Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, 27002 Lugo, Spain
The present work describes LC-ESI-MS/MS MS (liquid chromatography-electrospray ionization-tandem mass spectrometry) analyses of tryptic digestion peptides from phages that infect mastitis-causing Staphylococcus aureus isolated from dairy products. A total of 1933 nonredundant peptides belonging to 1282 proteins were identified and analyzed. Among them, 79 staphylococcal peptides from phages were confirmed. These peptides belong to proteins such as phage repressors, structural phage proteins, uncharacterized phage proteins and complement inhibitors. Moreover, eighteen of the phage origin peptides found were specific to S. aureus strains. These diagnostic peptides could be useful for the identification and characterization of S. aureus strains that cause mastitis. Furthermore, a study of bacteriophage phylogeny and the relationship among the identified phage peptides and the bacteria they infect was also performed. The results show the specific peptides that are present in closely related phages and the existing links between bacteriophage phylogeny and the respective Staphylococcus spp. infected.
