The aim of this study was to establish a regeneration system of watermelon. Watermelon W1 was selected as the experimental material using seedling shoots as a receptor. The effects of different concentrations of 6-Benzylaminopurine (6-BA) on the shoot-tip of watermelon seedlings were studied. Number of shoots at the stem tip were counted every other day until the new buds reached 2 cm. The new stem tip was cut, and the effect of different concentrations of Murashige and Skoog (MS) medium on the number of regenerated roots and root length of shoots were studied. The results showed that the differentiation rate was highest when the 6-BA concentration was 0.7 mg/mL to 0.8 mg/mL. The optimum concentration for root regeneration was 1/8 MS. At this concentration, the number rooted was the highest, and root length was also promoted.
