(Un)Tying the Knot: Oxidative Stress, Inflammatory Markers, and Lipid Status in Dogs with Hypercortisolism
Lazar Karić,
Filip Janjić,
Kristina Spariosu,
Darko Davitkov,
Vanja Krstić,
Milica Kovačević Filipović,
Milena Radaković
Affiliations
Lazar Karić
Department of Equine, Small Animal, Poultry and Wild Animal Diseases, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, Serbia
Filip Janjić
Department for Immunochemistry and Glycobiology, Institute for the Application of Nuclear Energy (INEP), University of Belgrade, Banatska 31b, 11000 Belgrade, Serbia
Kristina Spariosu
Department of Pathophysiology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobođenja 18, 11000 Belgrade, Serbia
Darko Davitkov
Department of Equine, Small Animal, Poultry and Wild Animal Diseases, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, Serbia
Vanja Krstić
Department of Equine, Small Animal, Poultry and Wild Animal Diseases, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, Serbia
Milica Kovačević Filipović
Department of Pathophysiology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobođenja 18, 11000 Belgrade, Serbia
Milena Radaković
Department of Pathophysiology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobođenja 18, 11000 Belgrade, Serbia
The aim of this study was to revisit the complex relationship between inflammation, oxidative stress and lipid metabolism in dogs with hypercortisolism (HC). Fourteen dogs newly diagnosed with HC and an equal number of healthy counterparts of similar age and markers of oxidative stress (AOPP, TBARS, TAC, GSH, PON-1, and UA) and inflammation (NLR, PLR, SII, HPT, CHE, CP, and Hcy) were included in the study. To determine the lipid profiles, cholesterol, and triglyceride levels were measured, and the electrophoretic separation of lipoproteins was performed. The results revealed that dogs with HC had higher levels of AOPP and TBARS, but only greater levels of GSH among antioxidants. Uric acid levels were higher in HC dogs, suggesting a pro-oxidative role. Elevated NLR, PLR, SII, and HPT levels were detected, but they did not seem to be associated with inflammation. Notable changes were detected in the HDL fraction, alongside hypercholesterolaemia and hypertriglyceridaemia. Correlation analysis revealed links between lipid markers and both oxidative stress and inflammatory indices. In conclusion, the data acquired may prove useful in further understanding of the intricate pathophysiology of Cushing’s syndrome.
