LncRNA HLA-F-AS1 Enhances the Migration, Invasion and Apoptosis of Glioblastoma Cells by Targeting lncRNA MEG3

Abstract

Yanhua Wang, Teng Xie, Huaming Liu, Xiaoping Yu Department of Neurosurgery, Hanchuan People’s Hospital, Hanchuan City, People’s Republic of ChinaCorrespondence: Xiaoping YuDepartment of Neurosurgery, Hanchuan People’s Hospital, No. 1 People’s Avenue, Hanchuan City, Hubei Province, 431600, People’s Republic of ChinaEmail [email protected]: Previous studies revealed the oncogenic role of long non-coding RNA (lncRNA) HLA-F-AS1 in colon cancer and breast cancer, while its role in other cancers is unclear. We predicted the direct interaction between HLA-F-AS1 and MEG3, which is a tumor suppressor lncRNA. We then assessed the interaction between HLA-F-AS1 and MEG3 in glioblastoma (GBM).Methods: The expression levels of HLA-F-AS1 and MEG3 in GBM and paired non-tumor tissues from 60 GBM patients were analyzed by RT-qPCR. Overexpression of HLA-F-AS1 and MEG3 was achieved in GBM cells to explore the interaction between them. The direct interaction between them was confirmed by RNA pull-down assay. The roles of HLA-F-AS1 and MEG3 in cell invasion, migration and apoptosis were explored by Transwell assays and cell apoptosis assay.Results: HLA-F-AS1 was highly expressed, and MEG3 was downregulated in GBM. Overexpression of HLA-F-AS1 reduced the expression levels of MEG3 while overexpression of MEG3 did not alter the expression of HLA-F-AS1. HLA-F-AS1 increased cell migration and invasion, but decreased cell apoptosis. MEG3 played opposite roles and reduced the effects of HLA-F-AS1 on cell behaviors.Conclusion: HLA-F-AS1 may sponge MEG3 in GBM cells to promote cell invasion and migration, and to suppress cell apoptosis.Keywords: glioblastoma, HLA-F-AS1, MEG3, invasion, migration, apoptosis

Keywords

• glioblastoma
• hla-f-as1
• meg3
• invasion
• migration
• apoptosis