Drought priming improves tolerance of Alhagi sparsifolia to subsequent drought: A coordinated interplay of phytohormones, osmolytes, and antioxidant potential
Abd Ullah,
Akash Tariq,
Fanjiang Zeng,
Muhammad Ahsan Asghar,
Jordi Sardans,
Corina Graciano,
Iftikhar Ali,
Josep Peñuelas
Affiliations
Abd Ullah
State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China; Xinjiang Key Laboratory of Desert Plant Roots Ecology and Vegetation Restoration, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China; Cele National Station of Observation and Research for Desert-Grassland Ecosystems, Cele 848300, China; University of Chinese Academy of Sciences, Beijing 100049, China
Akash Tariq
State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China; Xinjiang Key Laboratory of Desert Plant Roots Ecology and Vegetation Restoration, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China; Cele National Station of Observation and Research for Desert-Grassland Ecosystems, Cele 848300, China; University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding authors.
Fanjiang Zeng
State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China; Xinjiang Key Laboratory of Desert Plant Roots Ecology and Vegetation Restoration, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China; Cele National Station of Observation and Research for Desert-Grassland Ecosystems, Cele 848300, China; University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding authors.
Muhammad Ahsan Asghar
Department of Biological Resources, Agricultural Institute, Centre for Agricultural Research, ELKH, 2 13 Brunszvik St. Martonvásár, Hungary
Jordi Sardans
CSIC, Global Ecology Unit, CREAF-CSIC-UAB, Bellaterra, 08193 Barcelona, Catalonia, Spain; CREAF, Cerdanyola del Vallès 08193, Catalonia, Spain
Corina Graciano
Instituto de Fisiología Vegetal, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata, Buenos Aires, Argentina
Iftikhar Ali
Department of Genetics and Development, Columbia University Irving Medical Center, New York, United States of America
Josep Peñuelas
CSIC, Global Ecology Unit, CREAF-CSIC-UAB, Bellaterra, 08193 Barcelona, Catalonia, Spain; CREAF, Cerdanyola del Vallès 08193, Catalonia, Spain
Perennial trees are often stressed by drought more than once during their life cycle. Our study exposed three-month-old Alhagisparsifolia, with (drought-primed) or without (nonprimed) prior drought stress to subsequent drought for two months, aiming to reveal whether pre-exposure to drought could enhance seedling resistance to subsequent drought and investigated possible underlying mechanisms. Root biomass, leaf relative water content, chlorophyll a, and carotenoids were significantly higher in drought-primed than nonprimed seedlings. They also had reduced concentrations of malondialdehyde, hydrogen peroxide (H2O2), and superoxide anions (O2•−), indicating relief from oxidative stress. This relief was associated with a coordinated upregulation of enzymes scavenging O2•−and H2O2, particularly superoxide dismutase (SOD) and catalase (CAT), and the maintenance of the ascorbate-glutathione (AsA-GSH) redox pool and enzymatic activities (ascorbate peroxidase, mono- and dehydroascorbate reductase, and glutathione reductase), leading to the better regulation of reactive oxygen species. The failure of nonprimed seedlings to upregulate the SOD, CAT, and AsA-GSH cycles nevertheless made the seedlings susceptible to oxidative stress. The increased levels of strigolactones, jasmonic acid, and abscisic acid in drought-primed seedlings reveal their roles in subsequent stress. They also displayed higher gibberellic acid and indole acetic acid. A principal component analysis showed that the seedlings responded differently to drought if they had previously suffered a drought, mainly due to a higher capacity for pigment protection, oxidative scavenging, osmolytes, and anti-stress hormones. Our study provides insights into the benefits of stress memory induced in seedlings by early drought priming as a strategy for overcoming subsequent stress.
