A single-cell landscape of pre- and post-menopausal high-grade serous ovarian cancer ascites

Wenying Chen; Hanyuan Liu; Xinya Huang; Lili Qian; Liang Chen; Yonggang Zhou; Yi Liu; Yujie Liu; Yingying Wang; Tianjiao Zhang; Youyang Zhou; Jingwen Fang; Jiaxuan Yang; Fang Ni; Chuang Guo; Ying Zhou

iScience (Oct 2023)

A single-cell landscape of pre- and post-menopausal high-grade serous ovarian cancer ascites

Wenying Chen,
Hanyuan Liu,
Xinya Huang,
Lili Qian,
Liang Chen,
Yonggang Zhou,
Yi Liu,
Yujie Liu,
Yingying Wang,
Tianjiao Zhang,
Youyang Zhou,
Jingwen Fang,
Jiaxuan Yang,
Fang Ni,
Chuang Guo,
Ying Zhou

Affiliations

Wenying Chen: Department of Obstetrics and Gynecology, Core Facility Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China
Hanyuan Liu: Department of Obstetrics and Gynecology, Core Facility Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China
Xinya Huang: Department of Obstetrics and Gynecology, Core Facility Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China
Lili Qian: Department of Obstetrics and Gynecology, Core Facility Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China
Liang Chen: Department of Cardiology, The First Affiliated Hospital of USTC, the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, Anhui, China
Yonggang Zhou: The CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China; Institute of Immunology, University of Science and Technology of China, Hefei, China
Yi Liu: Department of Obstetrics and Gynecology, Core Facility Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China
Yujie Liu: Department of Obstetrics and Gynecology, Core Facility Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China
Yingying Wang: Department of Obstetrics and Gynecology, Core Facility Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China
Tianjiao Zhang: Department of Obstetrics and Gynecology, Core Facility Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China
Youyang Zhou: HanGene Biotech, Xiaoshan Innovation Polis, Hangzhou, Zhejiang 31200, China
Jingwen Fang: Department of Oncology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230021, China; HanGene Biotech, Xiaoshan Innovation Polis, Hangzhou, Zhejiang 31200, China
Jiaxuan Yang: HanGene Biotech, Xiaoshan Innovation Polis, Hangzhou, Zhejiang 31200, China
Fang Ni: The CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China; Institute of Immunology, University of Science and Technology of China, Hefei, China; Department of Hematology, The First Affiliated Hospital of USTC, Hefei, Anhui, China
Chuang Guo: Department of Oncology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230021, China; Department of Rheumatology and Immunology, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, Anhui, China; Corresponding author
Ying Zhou: Department of Obstetrics and Gynecology, Core Facility Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China; Corresponding author

Journal volume & issue: Vol. 26, no. 10
p. 107712

Abstract

Read online

Summary: High-grade serous ovarian cancer (HGSOC) is a hormone-related cancer with high mortality and poor prognosis. Based on the transcriptome of 57,444 cells in ascites from 10 patients with HGSOC (including 5 pre-menopausal and 5 post-menopausal patients), we identified 14 cell clusters which were further classified into 6 cell types, including T cells, B cells, NK cells, myeloid cells, epithelial cells, and stromal cells. We discovered an increased proportion of epithelial cells and a decreased proportion of T cells in pre-menopausal ascites compared with post-menopausal ascites. GO analysis revealed the pre-menopausal tumor microenvironments (TME) are closely associated with viral infection, while the post-menopausal TME are mostly related to the IL-17 immune pathway. SPP1/CD44-mediated crosstalk between myeloid cells and B cells, NK cells, and stromal cells mainly present in the pre-menopausal group, while SPP1/PTGER4 -mediated crosstalk between myeloid cells and epithelial cells mostly present in the post-menopausal group.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

About the journal

Abstract

Keywords