Biotecnología Vegetal (Oct 2004)
Evaluation of the phenotype variability and early selection to rust of the sugar cane (Puccinia melanocephala H. & P. Syd) in plants generated from irradiated callus from the variaty of sugar cane ´SP 70-1284´.
Abstract
It was evaluated the variability fenotípic in early phases of selection from plants regenerated of the mutagenic treatment with dose of 30 Gy of radiations Gamma (source 60Co) applicated to calluses in growth of the donating ´SP 70-1284´ tolerant to the rust disease of the sugarcane Puccinia melanocephala. The main changes the plants obtained in vitro, consinted in the height, the color, habit of growth, length and width of the leaf, as well as in the affectation for the rust disease. 14.28% of the changes all fenotipic was observed in plantín, and in the shoot the plants presented 10.99% of changes all fenotipic. In the vegetative multiplication one (MV1) three possible mutants were selected with smaller affectation in front rust disease of the sugarcan, resistance character to the rust disease efficiency of the tissue culture and the mutations induction with the dose of 30 Gy were of a mutant for each 1 525 plants evaluated in early phase of selection. Key words: variation fenotipic, selection early, mutagenesis