Generation of a human embryonic stem cell line targeted homozygous deletion of BMP10 (WAe007-A-2) by CRISPR/Cas9-dgRNA

Qian Li; Lianbo Shao; Luo Li; Han Shen; You Yu; Zhenya Shen

doi:10.1016/j.scr.2022.102942

Stem Cell Research (Dec 2022)

Generation of a human embryonic stem cell line targeted homozygous deletion of BMP10 (WAe007-A-2) by CRISPR/Cas9-dgRNA

Qian Li,
Lianbo Shao,
Luo Li,
Han Shen,
You Yu,
Zhenya Shen

Affiliations

Qian Li: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou 215123, Jiangsu, China; Suzhou Medical College, Soochow University, Suzhou 215123, Jiangsu, China
Lianbo Shao: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou 215123, Jiangsu, China; Suzhou Medical College, Soochow University, Suzhou 215123, Jiangsu, China
Luo Li: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou 215123, Jiangsu, China; Suzhou Medical College, Soochow University, Suzhou 215123, Jiangsu, China
Han Shen: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou 215123, Jiangsu, China; Suzhou Medical College, Soochow University, Suzhou 215123, Jiangsu, China
You Yu: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou 215123, Jiangsu, China; Suzhou Medical College, Soochow University, Suzhou 215123, Jiangsu, China; Corresponding authors at: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou 215123, Jiangsu, China.
Zhenya Shen: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou 215123, Jiangsu, China; Suzhou Medical College, Soochow University, Suzhou 215123, Jiangsu, China; Corresponding authors at: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou 215123, Jiangsu, China.

DOI: https://doi.org/10.1016/j.scr.2022.102942
Journal volume & issue: Vol. 65
p. 102942

Abstract

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BMP10 signaling has been implicated in regulation of cardiovascular cell fate determination and diseases, while the underlying molecular mechanism still remains uncertain. Here, the human embryonic stem cell line (H7-BMP10del) with homozygous deletion of BMP10 was generated by CRISPR/Cas9 method. Thus, the crosstalk related to BMP10 signaling could be investigated in cell fate determination and the molecular pathogenesis of cardiovascular disease.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.sciencedirect.com/journal/stem-cell-research

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