S. mutans Antisense vicK RNA Over-Expression Plus Antibacterial Dimethylaminohexadecyl Methacrylate Suppresses Oral Biofilms and Protects Enamel Hardness in Extracted Human Teeth

Shuang Yu; Mengmeng Xu; Zheng Wang; Yang Deng; Hockin H. K. Xu; Michael D. Weir; Negar Homayounfar; Guadalupe Garcia Fay; Hong Chen; Deqin Yang

doi:10.3390/pathogens13080707

Pathogens (Aug 2024)

S. mutans Antisense vicK RNA Over-Expression Plus Antibacterial Dimethylaminohexadecyl Methacrylate Suppresses Oral Biofilms and Protects Enamel Hardness in Extracted Human Teeth

Shuang Yu,
Mengmeng Xu,
Zheng Wang,
Yang Deng,
Hockin H. K. Xu,
Michael D. Weir,
Negar Homayounfar,
Guadalupe Garcia Fay,
Hong Chen,
Deqin Yang

Affiliations

Shuang Yu: Department of Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 404100, China
Mengmeng Xu: Department of Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 404100, China
Zheng Wang: Department of Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 404100, China
Yang Deng: Department of Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 404100, China
Hockin H. K. Xu: Department of Biomaterials and Regenerative Dental Medicine, University of Maryland Dental School, Baltimore, MD 21201, USA
Michael D. Weir: Department of Biomaterials and Regenerative Dental Medicine, University of Maryland Dental School, Baltimore, MD 21201, USA
Negar Homayounfar: Department of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD 21201, USA
Guadalupe Garcia Fay: Department of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD 21201, USA
Hong Chen: Department of Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 404100, China
Deqin Yang: Department of Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 404100, China

DOI: https://doi.org/10.3390/pathogens13080707
Journal volume & issue: Vol. 13, no. 8
p. 707

Abstract

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Streptococcus mutans (S. mutans) antisense vicK RNA (ASvicK) is a non-coding RNA that regulates cariogenic virulence and metabolic activity. Dimethylaminohexadecyl methacrylate (DMAHDM), a quaternary ammonium methacrylate used in dental materials, has strong antibacterial activity. This study examined the effects of S. mutans ASvicK on DMAHDM susceptibility and their combined impact on inhibiting S. mutans biofilm formation and protecting enamel hardness. The parent S. mutans UA159 and ASvicK overexpressing S. mutans (ASvicK) were tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentrations for planktonic bacteria (MBC-P) and biofilms (MBC-B) were measured. As the ASvicK MBC-B was 175 μg/mL, live/dead staining, metabolic activity (MTT), colony-forming units (CFUs), biofilm biomass, polysaccharide, and lactic acid production were investigated at 175 μg/mL and 87.5 μg/mL. The MIC, MBC-P, and MBC-B values for DMAHDM for the ASvicK strain were half those of the UA159 strain. In addition, combining S. mutans ASvicK with DMAHDM resulted in a significant 4-log CFU reduction (p p S. mutans ASvicK enhanced DMAHDM susceptibility, and their combination effectively inhibited biofilm formation and minimized enamel demineralization. The S. mutans ASvicK + DMAHDM combination shows great potential for anti-caries dental applications.

Published in Pathogens

ISSN: 2076-0817 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine
Website: http://www.mdpi.com/journal/pathogens

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