Morphological and genetic relationships of Myosotis laxa ssp. baltica and ssp. caespitosa, and typification of M. laxa ssp. baltica

Abstract

Intraspecific taxonomy of Myosotis laxa has been unclear for a long time. M. laxa ssp. baltica has been treated as a microendemic taxon of the Baltic Sea region, which has evolved in the Aland Islands and has spread northwards; the spread to southeast has been declared doubtful. Morphologically intermediate individuals between M. l. ssp. caespitosa and M. l. ssp. baltica exist; these have sometimes been classified as M. l. ssp. laxa. The aim of this paper is to clarify phylogenetic relationships between subspecies of M. laxa s.l. Here, M. laxa ssp. baltica is lectotypified. We proved that typical M. l. ssp. baltica does occur in the south-eastern Baltic region, namely in Estonia, using herbarium and freshly collected material. A group of plants were identified as typical M. l. ssp. baltica, but many specimens showed intermediate characters between M. laxa ssp. baltica and ssp. caespitosa. The two subspecies could be clearly differentiated neither by morphological characteristics nor by ITS sequences. M. laxa s. l. appeared to be monophyletic according to the ITS phylogeny. We propose that Myosotis laxa ssp. baltica is a coastal ecotype of Myosotis laxa, which has adapted to the fluctuating conditions of coastal habitats. Genetically, it has not yet evolved into a separate species and therefore it would be reasonable to assign it a rank of variety. However, further investigation including wider taxon and geographical sampling is needed to finally clarify the position of all subspecies.

Keywords

• Baltic Sea region
• phylogeny
• rDNA ITS
• microendemism
• taxonomy