Clinics (Apr 2014)

Resistance training improves body composition and increases matrix metalloproteinase 2 activity in biceps and gastrocnemius muscles of diet-induced obese rats

  • Markus Vinicius Campos Souza,
  • Richard Diego Leite,
  • Anderson Diogo de Souza Lino,
  • Rita de Cássia Marqueti,
  • Celene Fernandes Bernardes,
  • Heloisa Sobreiro Selistre de Araújo,
  • Eliete Bouskella,
  • Gilberto Eiji Shiguemoto,
  • Sérgio Eduardo de Andrade Perez,
  • Luiz Guilherme Kraemer-Aguiar

DOI
https://doi.org/10.6061/clinics/2014(04)08
Journal volume & issue
Vol. 69, no. 4
pp. 265 – 270

Abstract

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OBJECTIVE: We investigated the influence of resistance training on body composition and matrix metalloproteinase 2 activity in skeletal muscles of rats fed a high-fat diet. METHODS: Thirty-two Wistar rats were divided into four experimental groups (n = 8/each) according to diet and exercise status: Control (standard diet), Obese Control (high-fat diet), Resistance Training (standard diet) and Obese Resistance Training (high-fat diet) groups. Animals were fed a high-fat diet for 12 weeks to promote excessive weight gain. Resistance Training groups performed 12 weeks of training periods after this period in a vertical ladder three times/week. Fat percentage, fat-free mass and fat mass were assessed using dual-energy X-ray absorptiometry, and matrix metalloproteinase 2 activity in biceps and gastrocnemius muscles was analyzed using zymography. RESULTS: Resistance training significantly reduced body and fat masses and fat percentages in both trained groups (p<0.05). The maximal carrying load between trained groups was not different, but relative force was higher in the Resistance Training group (p<0.05). Of note, increased matrix metalloproteinase 2 activity was noted in the tested muscles of both trained groups (p<0.05). CONCLUSION: In conclusion, altered body composition and muscle matrix metalloproteinase 2 activity promoted by excessive weight gain were positively modified by resistance training.

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