Determinação de rutina em formulações farmacêuticas utilizando um sistema de análise por injeção em fluxo envolvendo multicomutação

Abstract

This paper reports a flow injection chemiluminescence method involving multicommutation for rutin determination in pharmaceutical formulations. The method is based on the oxidation of rutin by sodium hypochlorite which decreases the signal magnitude when compared with the same reaction without the rutin. Under optimized conditions, an analytical curve was obtained for rutin concentration range from 5.0 × 10-6 mol L-1 to 1.0 × 10-4 mol L-1 (r = 0.9966) with detection limit of 9.7 × 10-7 mol L-1 and a sampling frequency of 120 h-1. Repeatability study for a 7.0 × 10-6 mol L-1 rutine concentration showed a relative standard deviation (RSD) less than 5.0% for ten consecutives determination. The proposed method was applied successfully for rutin determination in pharmaceutical products.