Effect of forest management on the genetic diversity of Abies hidalgensis, a threatened species with restricted distribution

Abstract

Abies hidalgensis is an endemic species from the state of Hidalgo, Mexico, that has been registered only in nine fragmented relict populations that have a total of 1 000 individuals among them. Intensive forest management takes place in five of the populations under specific programs focused on Pinus spp. Still, it is necessary to know the impact of these activities on the genetic diversity of the threatened species, if restoration and conservation strategies are to be proposed. The aim of this work was to estimate the effect of forest management on the genetic structure of A. hidalgensis using seven nuclear molecular markers developed for A. guatemalensis (Ab07, Ab08, Ab09, Ab12, Ab15, Ab20, Ab23). The species was sampled growing under two different conditions; (i) areas under forest management and (ii) conserved areas. Two indexes of genetic diversity were evaluated, observed and expected heterozygosity. The genetic structure was determined by an analysis of molecular variance and a Bayesian assignment model. A bottleneck analysis was also carried out. The populations were found to have a common genetic base (differen­tiation coefficient FST = 0.056, number of mi­grants per generation Nm = 43), which suggests recent fragmentation of the distribution, which in turn increases the bottleneck effect in managed areas (Wilcoxon probability Wp = 0.007 and 0.016). This explains the apparently high heterozygous level (He = 0.69) and low inbreeding. Our results are important as they may be used to design strategies for management and conservation of A. hidalgensis.

Keywords

• bottleneck
• fir
• genetic structure
• genetic variation
• population genetics