Elucidation of the Potential Hair Growth-Promoting Effect of <i>Botryococcus terribilis</i>, Its Novel Compound Methylated-Meijicoccene, and C32 Botryococcene on Cultured Hair Follicle Dermal Papilla Cells Using DNA Microarray Gene Expression Analysis
Aprill Kee Oliva,
Meriem Bejaoui,
Atsushi Hirano,
Takashi Arimura,
Tran Ngoc Linh,
Eriko Uchiage,
Sachiko Nukaga,
Kenichi Tominaga,
Hiroyuki Nozaki,
Hiroko Isoda
Affiliations
Aprill Kee Oliva
Research and & Development Center for Tailor-Made QOL Program, University of Tsukuba, Tsukuba City 305-8550, Japan
Meriem Bejaoui
Research and & Development Center for Tailor-Made QOL Program, University of Tsukuba, Tsukuba City 305-8550, Japan
Atsushi Hirano
Tokyo Electric Power Company Holdings, Inc., 1-1-3 Uchisaiwai-cho, Chiyoda-ku, Tokyo 100-8560, Japan
Takashi Arimura
AIST-University of Tsukuba Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), AIST, University of Tsukuba, Tsukuba City 305-0006, Japan
Tran Ngoc Linh
AIST-University of Tsukuba Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), AIST, University of Tsukuba, Tsukuba City 305-0006, Japan
Eriko Uchiage
AIST-University of Tsukuba Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), AIST, University of Tsukuba, Tsukuba City 305-0006, Japan
Sachiko Nukaga
Tokyo Electric Power Company Holdings, Inc., 1-1-3 Uchisaiwai-cho, Chiyoda-ku, Tokyo 100-8560, Japan
Kenichi Tominaga
AIST-University of Tsukuba Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), AIST, University of Tsukuba, Tsukuba City 305-0006, Japan
Hiroyuki Nozaki
Tokyo Electric Power Company Holdings, Inc., 1-1-3 Uchisaiwai-cho, Chiyoda-ku, Tokyo 100-8560, Japan
Hiroko Isoda
Research and & Development Center for Tailor-Made QOL Program, University of Tsukuba, Tsukuba City 305-8550, Japan
A person’s quality of life can be adversely affected by hair loss. Microalgae are widely recognized for their abundance and rich functional components. Here, we evaluated the hair growth effect of a green alga, Botryococcus terribilis (B. terribilis), in vitro using hair follicle dermal papilla cells (HFDPCs). We isolated two types of cells from B. terribilis—green and orange cells, obtained from two different culture conditions. Microarray and real time-PCR results revealed that both cell types stimulated the expression of several pathways and genes associated with different aspect of the hair follicle cycle. Additionally, we demonstrated B. terribilis’ effect on collagen and keratin synthesis and inflammation reduction. We successfully isolated a novel compound, methylated-meijicoccene (me-meijicoccene), and C32 botryococcene from B. terribilis to validate their promising effects. Our study revealed that treatment with the two compounds had no cytotoxic effect on HFDPCs and significantly enhanced the gene expression levels of hair growth markers at low concentrations. Our study provides the first evidence of the underlying hair growth promoting effect of B. terribilis and its novel compound, me-meijicoccene, and C32 botryococcene.
