PLoS ONE (Jan 2018)

Automated sequential chromogenic IHC double staining with two HRP substrates.

  • Kenneth Heesche Petersen,
  • Jesper Lohse,
  • Lasse Ramsgaard

DOI
https://doi.org/10.1371/journal.pone.0207867
Journal volume & issue
Vol. 13, no. 11
p. e0207867

Abstract

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Automated IHC double staining using diaminobenzidine and HRP Magenta is illustrated utilizing a new acidic block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the first part of the double staining.