MALDI-TOF-MS for rapid screening analysis of M-protein in serum

Jie Li; Anping Xu; Weijie Xie; Bowen Li; Cunliang Yan; Yong Xia; Chao Liang; Chao Liang; Chao Liang; Ling Ji

doi:10.3389/fonc.2022.1073479

Frontiers in Oncology (Dec 2022)

MALDI-TOF-MS for rapid screening analysis of M-protein in serum

Jie Li,
Anping Xu,
Weijie Xie,
Bowen Li,
Cunliang Yan,
Yong Xia,
Chao Liang,
Chao Liang,
Chao Liang,
Ling Ji

Affiliations

Jie Li: Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China
Anping Xu: Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China
Weijie Xie: Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China
Bowen Li: Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China
Cunliang Yan: Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China
Yong Xia: Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China
Chao Liang: Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, China
Chao Liang: Institute of Integrated Bioinfomedicine and Translational Science (IBTS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, Hong Kong SAR, China
Chao Liang: State Key Laboratory of Proteomics, National Center for Protein Science (Beijing), Beijing Institute of Lifeomics, Beijing, China
Ling Ji: Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China

DOI: https://doi.org/10.3389/fonc.2022.1073479
Journal volume & issue: Vol. 12

Abstract

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Monoclonal immunoglobin (M-protein) is a serum biomarker for the diagnosis of plasma cell dyscrasias. Despite limitation of analytical sensitivity and resolution, serum protein electrophoresis and immunofixation electrophoresis are still the front-line tests for the detection of M-proteins. Herein, we developed a MALDI-TOF Mass spectrometry-based method for the screening test of M-proteins in human serum. Based on the unique mass signature of different immunoglobin isotypes, M-Proteins could be rapidly identified and typed. The method demonstrated with high analytical performance and throughput, rapid and simple, which could be a new choice for the diagnosis of plasma cell dyscrasias.

Published in Frontiers in Oncology

ISSN: 2234-943X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: https://www.frontiersin.org/journals/oncology/

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Abstract

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