THE STUDY OF LYMPHOCYTE APOPTOSIS INDUCED BY VIBRIO CHOLERAE ANTIGENS IN THE FORMATION OF IMMUNODEFICIENCY AND THE POSSIBILITY OF ITS CORRECTION

Abstract

Purpose: To study the role of lymphocyte apoptosis induced by Vibrio cholerae microbial cells and cholera toxin in the formation of the postvaccinal apoptotic immunodeficiency, as well as the possibility of its correction with the use of immunomodulator imunofan.Materials and methods: The apoptosis has been evaluated on the base of characteristic morphological changes of cells in preparations stained by histological stains, as well as by the method of flow cytofluorometry with propidium iodide staining on the cytofluorometer “NaviosТМ”.Results: It has been found that the etiological agent of cholera and its cholera toxin possess the apoptogenic activity towards murine T-lymphocytes. It has been shown that CD4+ T-cells are preferentially involved in the apoptosis. The role of scheduled cell death of immunocompetent cells in the formation of postvaccinal immunodeficiency in case of cholera is demonstrated. The preparation for immunocorrection of these changes is proposed.Summary: These findings confirm the usefulness of immunomodulator imunofan in vaccination against cholera and also open new perspectives for the improvement of cholera infection prophylaxis.

Keywords

• vibrio cholerae antigens
• lymphocytes
• apoptosis
• immunodeficiency
• imunophan immunomodulator