Comparison of Spatial Structures and Packaging of Phosphorybosil Pyrophosphate Synthetase 2 from <i>Thermus thermophilus</i> HB27 in Rhombohedral and Tetragonal Crystals
Yulia Abramchik,
Evgeniy Zayats,
Maria Kostromina,
Dmitry Lykoshin,
Ilya Fateev,
Irina Konstantinova,
Nadezda Zhukhlistova,
Vladimir Timofeev,
Inna Kuranova,
Roman Esipov
Affiliations
Yulia Abramchik
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str., 16/10, GSP-7, 117997 Moscow, Russia
Evgeniy Zayats
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str., 16/10, GSP-7, 117997 Moscow, Russia
Maria Kostromina
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str., 16/10, GSP-7, 117997 Moscow, Russia
Dmitry Lykoshin
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str., 16/10, GSP-7, 117997 Moscow, Russia
Ilya Fateev
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str., 16/10, GSP-7, 117997 Moscow, Russia
Irina Konstantinova
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str., 16/10, GSP-7, 117997 Moscow, Russia
Nadezda Zhukhlistova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Leninskii Prospect 59, 119333 Moscow, Russia
Vladimir Timofeev
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Leninskii Prospect 59, 119333 Moscow, Russia
Inna Kuranova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Leninskii Prospect 59, 119333 Moscow, Russia
Roman Esipov
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str., 16/10, GSP-7, 117997 Moscow, Russia
We report the spatial structure of phosphoribosyl pyrophosphate synthetase 2 from the thermophilic bacterium Thermus thermophilus HB27 (TthPRPPS2) obtained at a 1.85 Å resolution using a diffraction set collected from rhombohedral crystals (space group R32-h), grown with lithium sulfate as a precipitant. This crystal structure was compared with the structure of TthPRPPS2, previously obtained at a 2.2 Å resolution using diffraction sets from the tetragonal crystals (space group P41212), grown with ammonium sulfate as a precipitant. The comparison of these structures allows the study of the differences between protein molecules in both crystalline structures, as well as the packaging of enzyme molecules in crystals of both spatial groups. Our results may contribute to the research of the structural basis of catalytic activity and substrate specificity of this enzyme.
