Systemic neutrophil migration and rapid consumption of neutrophils in the spleen
Yuji Takeda,
Tomoyuki Kato,
Nobuhito Nemoto,
Akemi Araki,
Mohammad Yeashin Gazi,
Hidetoshi Nara,
Hironobu Asao
Affiliations
Yuji Takeda
Department of Immunology, Yamagata University Faculty of Medicine, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan
Tomoyuki Kato
Department of Urology, Yamagata University Faculty of Medicine, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan
Nobuhito Nemoto
Department of Immunology, Yamagata University Faculty of Medicine, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan; Department of Orthopaedics, Yamagata University Faculty of Medicine, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan
Akemi Araki
Department of Immunology, Yamagata University Faculty of Medicine, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan
Mohammad Yeashin Gazi
Department of Immunology, Yamagata University Faculty of Medicine, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan
Hidetoshi Nara
Department of Immunology, Yamagata University Faculty of Medicine, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan
Hironobu Asao
Department of Immunology, Yamagata University Faculty of Medicine, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan; Corresponding author.
The systemic migration of neutrophils is not fully understood. In this study, we purified neutrophils from rat peripheral blood and labeled them with [51Cr] sodium chromate. The labeled cells were injected into the tail veins of rats, and were traced. Neutrophils were rapidly trapped in the liver and the spleen within 6 h. The migration ratios of neutrophils in the lung and the gut were lower compared with those in the liver and the spleen. Interestingly, migrated cells into the spleen were rapidly phagocytosed by monocytes/macrophages. Therefore, accumulation of intact neutrophils in the spleen may be difficult to measure.
