OncoTargets and Therapy (Oct 2019)

The Long Non-Coding RNA SBF2-AS1 Exerts Oncogenic Functions In Gastric Cancer By Targeting The miR-302b-3p/E2F Transcription Factor 3 Axis

  • Liang C,
  • Yue C,
  • Liang C,
  • Ge H,
  • Wei Z,
  • Li G,
  • Wu J,
  • Huang H,
  • Guo J

Journal volume & issue
Vol. Volume 12
pp. 8879 – 8893

Abstract

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Chaojie Liang,1,* Chaosen Yue,2,* Chaowei Liang,1 Hua Ge,2 Zhigang Wei,1 Guangming Li,2 Jixiang Wu,2 He Huang,1 Jiansheng Guo1 1Department of General Surgery, First Hospital/First Clinical Medical College of Shanxi Medical University, Taiyuan, Shanxi 030001, People’s Republic of China; 2Department of General Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jiansheng Guo; He HuangDepartment of General Surgery, First Hospital/First Clinical Medical College of Shanxi Medical University, Taiyuan, Shanxi 030001 People’s Republic of ChinaTel +86 150 3515 8467; +86 188 0126 1267Email [email protected]; [email protected] and aims: Studies show that the long non-coding RNA, SBF2-AS1, plays a critical role in cancer progression, but the role of SBF2-AS1 in gastric cancer has not been reported. Therefore, this study aimed to elucidate the mechanism of SBF2-AS1 in gastric cancer (GC).Methods: A meta-analysis, based on the gene expression omnibus database and TCGA dataset was performed to explore the prognostic value of SBF2-AS1 in GC. RT-PCR was also conducted to investigate the clinicopathologic value of SBF2-AS1 in GC. The effect of SBF2-AS1 in GC cell lines was conducted by gain or loss-of-function assays, and the SBF2-AS1 target gene was confirmed using a luciferase reporter assay and bioinformatics.Results: SBF2-AS1 was overexpressed in GC tissues and cell lines, and SBF2-AS1 overexpression indicated poor overall survival and could serve as an independent prognostic factor. Moreover, knockdown of SBF2-AS1 inhibited cell growth, invasion, and metastasis, promoted apoptosis, and caused cell cycle arrest. Luciferase reporter and gain- or loss-of-function assays indicated that SBF2-AS1 acted as a competing endogenous (ceRNA) for microRNA (miR)-302b-3p, which blocked the inhibitory effect of miR-302b-3p on the E2F transcription factor 3 (E2F3).Conclusion: SBF2-AS1 could be a potential diagnostic and prognostic biomarker in GC, and SBF2-AS1 accelerates tumor progression via the miR-302b-3p/E2F3 axis.Keywords: SBF2-AS1, gastric cancer, miR-302b-3p, E2F3

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