Pinus roxburghii and Nauplius graveolens Extracts Elevate Apoptotic Gene Markers in C26 Colon Carcinoma Cells Induced in a BALB/c Mouse Model

Mosab Gad; Hassan Z. Hassouna; Khaled Mahmoud; Ahmed A. Abd-Rabou; Amal S. Abdel-Azeem; Amany M. Hegazy; Mohamed S. Abdel-Lattife; Fouad A. Ahmed; Fatih Oz; Charalampos Proestos; Ahmed A. Zaky

doi:10.3390/separations9100277

Separations (Oct 2022)

Pinus roxburghii and Nauplius graveolens Extracts Elevate Apoptotic Gene Markers in C26 Colon Carcinoma Cells Induced in a BALB/c Mouse Model

Mosab Gad,
Hassan Z. Hassouna,
Khaled Mahmoud,
Ahmed A. Abd-Rabou,
Amal S. Abdel-Azeem,
Amany M. Hegazy,
Mohamed S. Abdel-Lattife,
Fouad A. Ahmed,
Fatih Oz,
Charalampos Proestos,
Ahmed A. Zaky

Affiliations

Mosab Gad: Nutrition and Food Science Department, Food Industries and Nutrition Research Institute, National Research Centre, Cairo 12622, Egypt
Hassan Z. Hassouna: Nutrition and Food Science Department, Food Industries and Nutrition Research Institute, National Research Centre, Cairo 12622, Egypt
Khaled Mahmoud: Pharmacognosy Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Cairo 12622, Egypt
Ahmed A. Abd-Rabou: Hormones Department, Medical Research and Clinical Studies Institute, National Research Centre, Cairo 12622, Egypt
Amal S. Abdel-Azeem: Nutrition and Food Science Department, Food Industries and Nutrition Research Institute, National Research Centre, Cairo 12622, Egypt
Amany M. Hegazy: Nutrition and Food Science Department, Food Industries and Nutrition Research Institute, National Research Centre, Cairo 12622, Egypt
Mohamed S. Abdel-Lattife: Biochemistry Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt
Fouad A. Ahmed: Biochemistry Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt
Fatih Oz: Department of Food Engineering, Faculty of Agriculture, Ataturk University, Erzurum 25240, Türkiye
Charalampos Proestos: Laboratory of Food Chemistry, Department of Chemistry, National and Kapodistrian University of Athens Zografou, 15771 Athens, Greece
Ahmed A. Zaky: Department of Food Technology, Food Industries and Nutrition Research Institute, National Research Centre, Dokki, Cairo 12622, Egypt

DOI: https://doi.org/10.3390/separations9100277
Journal volume & issue: Vol. 9, no. 10
p. 277

Abstract

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The present study aimed to evaluate the chemopreventive potential of Pinus roxburghii branch (P. roxburghii) and Nauplius graveolens (N. graveolens) extracts against human colorectal cancer (CRC) induced by C26 murine cells in a BALB/c mouse model. Real-time qRT-PCR was used to evaluate the apoptotic pathway by measuring the relative mRNA expression levels of the Bcl-2, Bax, Cas3, NF-κB, and PI3k genes. At the termination of the 30-day period, blood samples were collected to assay the biomarkers. The results showed a significant increase (p P. roxburghii and N. graveolens extracts. Administration of one-tenth of the LD50 of P. roxburghii and N. graveolens extracts caused a significant upregulation of the expression of Bax and Cas-3 and downregulation of the Bcl-2, NF-ĸB, and PI3k genes vs. the GAPDH gene as a housekeeping gene compared to the control group. Furthermore, the Bax/Bcl-2 ratio increased upon treatment. After administration of P. roxburghii and N. graveolens at a safe dose (1/10) of a lethal dose, the results showed improvement in both body weight gain and a significant decrease (p P. roxburghii and N. graveolens extracts can be utilized as potential chemopreventive agents for CRC treatment by stimulating cancer cell apoptosis and suppressing CRC survival and proliferation.

Published in Separations

ISSN: 2297-8739 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Physics; Science: Chemistry
Website: http://www.mdpi.com/journal/separations

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