Aquaculture performance of artificial interspecific backcross groups between the hybrid of Crassostrea hongkongensis × C. ariakensis and the two parental species

Yanping Qin; Qinggan Xing; Xingyou Li; Jiangwei Li; Weitao Wan; Chao Yue; Haitao Ma; Jun Li; Ying Pan; Ziniu Yu; Yuehuan Zhang

doi:10.1016/j.aqrep.2025.102926

Aquaculture Reports (Sep 2025)

Aquaculture performance of artificial interspecific backcross groups between the hybrid of Crassostrea hongkongensis × C. ariakensis and the two parental species

Yanping Qin,
Qinggan Xing,
Xingyou Li,
Jiangwei Li,
Weitao Wan,
Chao Yue,
Haitao Ma,
Jun Li,
Ying Pan,
Ziniu Yu,
Yuehuan Zhang

Affiliations

Yanping Qin: Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; College of Animal Science and Technology, Guangxi University, Nanning 530004, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Research Institute of Marine Ecological Environment Engineering, Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Shenzhen 518124, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519015, China; Agro-Tech Extension Center of Guangdong Province, Guangzhou 510145, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; University of Chinese Academy of Sciences, Beijing 100049, China
Qinggan Xing: Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; College of Animal Science and Technology, Guangxi University, Nanning 530004, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Research Institute of Marine Ecological Environment Engineering, Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Shenzhen 518124, China
Xingyou Li: Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; College of Animal Science and Technology, Guangxi University, Nanning 530004, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Research Institute of Marine Ecological Environment Engineering, Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Shenzhen 518124, China; University of Chinese Academy of Sciences, Beijing 100049, China
Jiangwei Li: Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; College of Animal Science and Technology, Guangxi University, Nanning 530004, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Research Institute of Marine Ecological Environment Engineering, Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Shenzhen 518124, China
Weitao Wan: Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; College of Animal Science and Technology, Guangxi University, Nanning 530004, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Research Institute of Marine Ecological Environment Engineering, Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Shenzhen 518124, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519015, China; Agro-Tech Extension Center of Guangdong Province, Guangzhou 510145, China; University of Chinese Academy of Sciences, Beijing 100049, China
Chao Yue: Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; College of Animal Science and Technology, Guangxi University, Nanning 530004, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Research Institute of Marine Ecological Environment Engineering, Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Shenzhen 518124, China; University of Chinese Academy of Sciences, Beijing 100049, China
Haitao Ma: Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; College of Animal Science and Technology, Guangxi University, Nanning 530004, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Research Institute of Marine Ecological Environment Engineering, Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Shenzhen 518124, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519015, China; Agro-Tech Extension Center of Guangdong Province, Guangzhou 510145, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China
Jun Li: Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; College of Animal Science and Technology, Guangxi University, Nanning 530004, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Research Institute of Marine Ecological Environment Engineering, Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Shenzhen 518124, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519015, China; Agro-Tech Extension Center of Guangdong Province, Guangzhou 510145, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China
Ying Pan: Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Ziniu Yu: Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; College of Animal Science and Technology, Guangxi University, Nanning 530004, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Research Institute of Marine Ecological Environment Engineering, Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Shenzhen 518124, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519015, China; Agro-Tech Extension Center of Guangdong Province, Guangzhou 510145, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; University of Chinese Academy of Sciences, Beijing 100049, China; Correspondence to: South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
Yuehuan Zhang: Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; College of Animal Science and Technology, Guangxi University, Nanning 530004, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Research Institute of Marine Ecological Environment Engineering, Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Shenzhen 518124, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519015, China; Agro-Tech Extension Center of Guangdong Province, Guangzhou 510145, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; University of Chinese Academy of Sciences, Beijing 100049, China; Correspondence to: South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.

DOI: https://doi.org/10.1016/j.aqrep.2025.102926
Journal volume & issue: Vol. 43
p. 102926

Abstract

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Crassostrea hongkongensis is popular in southern China for its white flesh color, unique flavor, and delicious taste. However, the disadvantages of slow growth, poor tolerance, and high mortality limit its production and market sales. The application of genetic breeding techniques to breed new breeding lines with fast growth and low mortality is an important approach to solve these industrial challenges, including interspecific backcross breeding. In this study, we established eight experimental groups (HH-C. hongkonggensis ♀ × C. hongkonggensis ♂; HA-C. hongkonggensis ♀ × C. ariakensis♂; AA-C. ariakensis ♂ × C. ariakensis; A/HA-C. ariakensis♀ × HA ♂; HA/HA-HA ♀ × HA ♂; H/HA-C. hongkonggensis ♀ × HA ♂; HA/H- HA ♀ × C. hongkonggensis♂ and HA/A-HA ♀ × C. ariakensis ♂), tracked and compared their phenotypic traits. The results confirmed that the hatching parameters of the interspecific backcross groups (HA/H, HA/A, H/HA and A/HA) were significantly lower than those of the pure parental progeny groups (HH and AA). Although HA/A did not always show a significant growth advantage in the larval stage, it exhibited the largest shell height during the marine culture period (except on the 210th day), and showed a significant shell height advantage on the 360th day (P < 0.05). Moreover, there were obvious backcross advantages during the marine culture period, with backcross advantages (BAs) in shell height of 7.30 %, 17.66 %, 16.44 % and 10.95 % from the 90th to the 360th days, respectively. In addition, HA/A consistently ranked in the top three in terms of the incremental survival rate, being significantly higher than the grandparental offsprings (HH and AA), with HA/A showing the highest survival rates on the 210th and 360th days. Similarly, backcross advantages were observed at all time points, with BAs of 3.42 %, 5.96 %, 6.29 % and 0.43 %, respectively. Gonad development statistics and paraffin section results showed that all backcross groups were fertile and produced many normal gametes, with little difference in the ratios of males to females among the eight groups. In summary, the HA/A group showed significant backcross advantages in both growth and survival, with the best overall performance. This makes it a promising new strain that could help address the industrial problems of C. hongkongensis and has the potential for large-scale application.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: https://www.sciencedirect.com/journal/aquaculture-reports

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