Microdetermination of cholesterol in serum lipoproteins

Abstract

A two-step procedure for the microdetermination of cholesterol in serum lipoproteins is compared with cholesterol quantitation after density gradient ultracentrifugation. Serum lipoproteins from 10 mul of serum are separated by electrophoresis on agarose and visualized by precipitation with dextran sulfate—CaCl2. The lipoprotein bands are cut off from the plates, the agarose slices are hydrolyzed by gas-liquid chromatography. The comparison between the two procedures reveals satisfactory correlations for β-and pre-β-lipoproteins and total serum. There is excellent recovery of cholesterol in fractionated lipoproteins.

Keywords

• lipoprotein electrophoresis
• gasliquid chromatography