Spolehlivost záchytu virů jádrovin a viru šarky švestky metodou ELISA v zimním období [Reliability of Pome viruses and Plum pox virus detection during the wintertime using an ELISA method]

Abstract

Apple mosaic virus (ApMV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), and Apple stem grooving virus (ASGV), and together with Plum pox virus (PPV) are regularly monitored worldwide in fruit trees. These viruses are usually routinely tested using a serological method enzyme linked immunosorbent assays (ELISA). However, the suitability of the ELISA method for virus testing throughout the entire year is limited by an inconsistent reliability of virus detections in plant tissues during seasons. Our work focused on suitability of plant tissues sampled during the wintertime for the detection of the apple viruses and PPV using ELISA. Shoots of infected apple and plum trees were sampled in four terms in January, and either dormant buds or sprouting buds were used for the testing. We found that ACLSV, ApMV, and ASPV could be reliably detected in buds after 7 days of sprouting. However, 14 days of sprouting was necessary for ASGV. Ideally, sampling should be carried out in the second half of January. PPV was reliably detected during the entire January from dormant buds without any need of forced sprouting.

Keywords

• aclsv
• apmv
• aspv
• asgv
• ppv
• elisa detection
• buds