Phenotypic and Molecular Detection of Pathogenic Vibrio Species in Two Different Regions of the Caspian Sea in Mazandaran, Iran

Abstract

Background : Vibrio species including V. cholera, V. mimicus, V. parahaemolyticus, and V.vulnificus may cause gastrointestinal diseases after seafood consumption, and wound infections in swimmers and fishermen after exposing to seawater. This study determined the prevalence of the four Vibrio species in Tonkabon and Ramsar recreational beach water (approximately 200 meter far from the place where the river reaches the sea) and estuaria (place where the river reaches the sea) in northern part of Iran from autumn 1391 through autumn 1392.Methods : Three hundreds water samples were collected for the detection of Vibrio species,using biochemical identification.Results : Genomic DNA extracted from isolates and 16Sr DNA PCR confirmed the successful isolation of 9 vibrio species in recreational beach water region.Conclusions : Out of 300 samples, nine positive samples include one V. cholerae and eight V.parahaemolyticus were found at recreational beach (approximately 200 meter far from the place where the river reaches the sea).

Keywords

• Pathogenic Vibrio, species, Caspian Sea, 16S rDNA PCR