Nature Communications (May 2018)

Programmable sequential mutagenesis by inducible Cpf1 crRNA array inversion

  • Ryan D. Chow,
  • Hyunu Ray Kim,
  • Sidi Chen

DOI
https://doi.org/10.1038/s41467-018-04158-z
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 9

Abstract

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Few models recapitulate the stepwise nature of cancer evolution. Here the authors introduce Cpf1-Flip, a system to introduce mutations sequentially through inducible inversion of a crRNA array.