INDUCTION AND DIFFERENTIATION OF CALLUS FROM TERMINAL DORMANT BUDS AND ROOTS OF Gladiolus hybrida

Abstract

The present study was carried out in October . ( 2008 ) to April ( 2009 ), of Gladiolus plants " White prosperity " cv. terminal dormant buds length 0.5cm cultured on MS medium supplemented with NAA , 2,4-D , TDZ , BA . or 1cm part of root produced in vitro cultured in MS medium supplemented with different growth regulator for callus induction and differentiation , data taken after 10 weeks . Data refers , highest percentage for callus formation was obtained from culture dormant terminal buds on MS medium supplemented with 6 mg/L NAA and this gave large amount of callus which need 35 days for induction , these callus cultured on MS medium free from hormones as control or supplemented with ( 1 mg/L kin + 0.1 mg/L NAA ) or (2 mg/L kin + 0.2 mg/L NAA ) the treatment at (2 mg/L kin + 0.2 mg/L NAA ) gave significant effect for all parameter , high percentage 80% for callus differentiation and highest number of shoot 9 shoot / explant with highest shoot length 5.5 cm and highest number of root 15 root / explant with longer root 4.5 cm , callus cultured on control treatment did not differentiation . Callus obtained with percentage 70-90 from cultured parts of root on MS medium supplemented with (2 mg/L 2,4-D + o.6 mg/L NAA ) , (5 mg/L 2,4-D + 2 mg/L kin ) , (1 mg/L NAA + 1 mg/L kin ) . Callus produced from (5 mg/L 2,4-D + o.6 mg/L kin ) treatment cultured on MS medium supplemented with ( 0.0 , 1 mg/L kin , 2 mg/L BA ) and this callus gave highest percentage 80% of shoots production from cultured on MS medium supplemented with 2 mg/L BA and highest shoot lengths 5 cm and highest number of root 16 root / explant and 5.5 cm length . shoots produced from Callus , cultured on MS medium with 1 mg/L IBA to rooting after that transport to laboratory to grow normally with survival 100%.