Live-cell imaging reveals the relative contributions of antigen-presenting cell subsets to thymic central tolerance

J. N. Lancaster; H. M. Thyagarajan; J. Srinivasan; Y. Li; Z. Hu; L. I. R. Ehrlich

doi:10.1038/s41467-019-09727-4

Nature Communications (May 2019)

Live-cell imaging reveals the relative contributions of antigen-presenting cell subsets to thymic central tolerance

J. N. Lancaster,
H. M. Thyagarajan,
J. Srinivasan,
Y. Li,
Z. Hu,
L. I. R. Ehrlich

Affiliations

J. N. Lancaster: Department of Molecular Biosciences, Institute of Cellular and Molecular Biology, The University of Texas at Austin
H. M. Thyagarajan: Department of Molecular Biosciences, Institute of Cellular and Molecular Biology, The University of Texas at Austin
J. Srinivasan: Department of Molecular Biosciences, Institute of Cellular and Molecular Biology, The University of Texas at Austin
Y. Li: Department of Molecular Biosciences, Institute of Cellular and Molecular Biology, The University of Texas at Austin
Z. Hu: Department of Molecular Biosciences, Institute of Cellular and Molecular Biology, The University of Texas at Austin
L. I. R. Ehrlich: Department of Molecular Biosciences, Institute of Cellular and Molecular Biology, The University of Texas at Austin

DOI: https://doi.org/10.1038/s41467-019-09727-4
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 15

Abstract

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Both thymic epithelial cells and dendritic cells present self antigens in the thymus to mediate thymic selection and T cell tolerance. Here the authors quantify, using two-photon live imaging of mouse thymic slices, the relative contribution of these two cell types, as well as the effects of antigen cross-presentation by dendritic cells, during tolerance induction.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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