Synthesis, Characterization, and Evaluation of the Antimicrobial Effects and Cytotoxicity of a Novel Nanocomposite Based on Polyamide 6 and Trimetaphosphate Nanoparticles Decorated with Silver Nanoparticles
Leonardo Antônio de Morais,
Francisco Nunes de Souza Neto,
Thayse Yumi Hosida,
Danilo Martins dos Santos,
Bianca Carvalho de Almeida,
Elisabete Frollini,
Sergio Paulo Campana Filho,
Debora de Barros Barbosa,
Emerson Rodrigues de Camargo,
Alberto Carlos Botazzo Delbem
Affiliations
Leonardo Antônio de Morais
Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, São Paulo, Brazil
Francisco Nunes de Souza Neto
Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, São Paulo, Brazil
Thayse Yumi Hosida
Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, São Paulo, Brazil
Danilo Martins dos Santos
Sao Carlos Institute of Chemistry, University of Sao Paulo, Av. Trabalhador Sao-Carlense, 400, São Carlos 13566-590, São Paulo, Brazil
Bianca Carvalho de Almeida
Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, São Paulo, Brazil
Elisabete Frollini
Sao Carlos Institute of Chemistry, University of Sao Paulo, Av. Trabalhador Sao-Carlense, 400, São Carlos 13566-590, São Paulo, Brazil
Sergio Paulo Campana Filho
Sao Carlos Institute of Chemistry, University of Sao Paulo, Av. Trabalhador Sao-Carlense, 400, São Carlos 13566-590, São Paulo, Brazil
Debora de Barros Barbosa
Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, São Paulo, Brazil
Emerson Rodrigues de Camargo
Center for Exact Sciences and Technology, Federal University of São Carlos (UFSCAR), Av. Trab. São Carlense, 400, São Carlos 13566-590, São Paulo, Brazil
Alberto Carlos Botazzo Delbem
Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, São Paulo, Brazil
This study aimed to develop a polymeric matrix of polyamide-6 (P6) impregnated with trimetaphosphate (TMP) nanoparticles and silver nanoparticles (AgNPs), and to evaluate its antimicrobial activity, surface free energy, TMP and Ag+ release, and cytotoxicity for use as a support in dental tissue. The data were subjected to statistical analysis (p + was released for all groups decorated with AgNPs, and for TMP, the release only occurred for the P6-TMP-5% and P6-TMP-10% groups. In the inhibition zones, the AgNPs showed activity against both microorganisms. The P6-TMP-2.5%-Ag and P6-TMP-5%-Ag groups with AgNPs showed a greater reduction in CFU for S. mutans. For C. albicans, all groups showed a reduction in CFU. The P6-TMP groups showed higher cell viability, regardless of time (p < 0.05). The developed P6 polymeric matrix impregnated with TMP and AgNPs demonstrated promising antimicrobial properties against the tested microorganisms, making it a potential material for applications in scaffolds in dental tissues.
