In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa

Luis F. Avila-Cobian; Stefania De Benedetti; Choon Kim; Rhona Feltzer; Matthew M. Champion; Jed F. Fisher; Shahriar Mobashery

doi:10.1038/s42003-022-04230-x

Communications Biology (Nov 2022)

In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa

Luis F. Avila-Cobian,
Stefania De Benedetti,
Choon Kim,
Rhona Feltzer,
Matthew M. Champion,
Jed F. Fisher,
Shahriar Mobashery

Affiliations

Luis F. Avila-Cobian: Department of Chemistry and Biochemistry, University of Notre Dame
Stefania De Benedetti: Department of Chemistry and Biochemistry, University of Notre Dame
Choon Kim: Department of Chemistry and Biochemistry, University of Notre Dame
Rhona Feltzer: Department of Chemistry and Biochemistry, University of Notre Dame
Matthew M. Champion: Department of Chemistry and Biochemistry, University of Notre Dame
Jed F. Fisher: Department of Chemistry and Biochemistry, University of Notre Dame
Shahriar Mobashery: Department of Chemistry and Biochemistry, University of Notre Dame

DOI: https://doi.org/10.1038/s42003-022-04230-x
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 16

Abstract

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An extensive protein-interaction network of cell-wall biosynthesis assemblies in P. aeruginosa is elucidated using a pulldown strategy with mass-spectrometry-based proteomics and binding assays.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal