Simultaneous deep transcriptome and proteome profiling in a single mouse oocyte
Yi-Rong Jiang,
Le Zhu,
Lan-Rui Cao,
Qiong Wu,
Jian-Bo Chen,
Yu Wang,
Jie Wu,
Tian-Yu Zhang,
Zhao-Lun Wang,
Zhi-Ying Guan,
Qin-Qin Xu,
Qian-Xi Fan,
Shao-Wen Shi,
Hui-Feng Wang,
Jian-Zhang Pan,
Xu-Dong Fu,
Yongcheng Wang,
Qun Fang
Affiliations
Yi-Rong Jiang
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China
Le Zhu
School of Medicine, Liangzhu Laboratory, Zhejiang University, Hangzhou 311113, China
Lan-Rui Cao
School of Medicine, Liangzhu Laboratory, Zhejiang University, Hangzhou 311113, China
Qiong Wu
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China
Jian-Bo Chen
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China
Yu Wang
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou 311200, China
Jie Wu
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China
Tian-Yu Zhang
M20 Genomics, Hangzhou 311100, China
Zhao-Lun Wang
M20 Genomics, Hangzhou 311100, China
Zhi-Ying Guan
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China
Qin-Qin Xu
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China
Qian-Xi Fan
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China
Shao-Wen Shi
ZJU-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou 311200, China
Hui-Feng Wang
ZJU-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou 311200, China
Jian-Zhang Pan
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou 311200, China
Xu-Dong Fu
School of Medicine, Liangzhu Laboratory, Zhejiang University, Hangzhou 311113, China; Center of Stem Cell and Regenerative Medicine and Bone Marrow Transplantation Center of the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310011, China; Corresponding author
Yongcheng Wang
School of Medicine, Liangzhu Laboratory, Zhejiang University, Hangzhou 311113, China; Department of Laboratory Medicine, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310011, China; Corresponding author
Qun Fang
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou 311200, China; Key Laboratory of Excited-State Materials of Zhejiang Province, Zhejiang University, Hangzhou 310007, China; Corresponding author
Summary: Although single-cell multi-omics technologies are undergoing rapid development, simultaneous transcriptome and proteome analysis of a single-cell individual still faces great challenges. Here, we developed a single-cell simultaneous transcriptome and proteome (scSTAP) analysis platform based on microfluidics, high-throughput sequencing, and mass spectrometry technology to achieve deep and joint quantitative analysis of transcriptome and proteome at the single-cell level, providing an important resource for understanding the relationship between transcription and translation in cells. This platform was applied to analyze single mouse oocytes at different meiotic maturation stages, reaching an average quantification depth of 19,948 genes and 2,663 protein groups in single mouse oocytes. In particular, we analyzed the correlation of individual RNA and protein pairs, as well as the meiosis regulatory network with unprecedented depth, and identified 30 transcript-protein pairs as specific oocyte maturational signatures, which could be productive for exploring transcriptional and translational regulatory features during oocyte meiosis.
