Microwave Spectroscopic Detection of Human Hsp70 Protein on Annealed Gold Nanostructures on ITO Glass Strips
Rodica Elena Ionescu,
Raphael Selon,
Nicolas Pocholle,
Lan Zhou,
Anna Rumyantseva,
Eric Bourillot,
Eric Lesniewska
Affiliations
Rodica Elena Ionescu
Light, Nanomaterials and Nanotechnology (L2N), FRE-CNRS 2019, Institute Charles Delaunay (ICD), University of Technology of Troyes, 12 Rue Marie Curie CS 42060, 10004 Troyes CEDEX, France
Raphael Selon
Laboratory Interdisciplinaire Carnot de Bourgogne (ICB), UMR-CNRS 6303, University of Bourgogne Franche-Comté, 9 Avenue Alain Savary, 21078 Dijon CEDEX, France
Nicolas Pocholle
Laboratory Interdisciplinaire Carnot de Bourgogne (ICB), UMR-CNRS 6303, University of Bourgogne Franche-Comté, 9 Avenue Alain Savary, 21078 Dijon CEDEX, France
Lan Zhou
Light, Nanomaterials and Nanotechnology (L2N), FRE-CNRS 2019, Institute Charles Delaunay (ICD), University of Technology of Troyes, 12 Rue Marie Curie CS 42060, 10004 Troyes CEDEX, France
Anna Rumyantseva
Light, Nanomaterials and Nanotechnology (L2N), FRE-CNRS 2019, Institute Charles Delaunay (ICD), University of Technology of Troyes, 12 Rue Marie Curie CS 42060, 10004 Troyes CEDEX, France
Eric Bourillot
Laboratory Interdisciplinaire Carnot de Bourgogne (ICB), UMR-CNRS 6303, University of Bourgogne Franche-Comté, 9 Avenue Alain Savary, 21078 Dijon CEDEX, France
Eric Lesniewska
Laboratory Interdisciplinaire Carnot de Bourgogne (ICB), UMR-CNRS 6303, University of Bourgogne Franche-Comté, 9 Avenue Alain Savary, 21078 Dijon CEDEX, France
Conductive indium-tin oxide (ITO) and non-conductive glass substrates were successfully modified with embedded gold nanoparticles (AuNPs) formed by controlled thermal annealing at 550 °C for 8 h in a preselected oven. The authors characterized the formation of AuNPs using two microscopic techniques: scanning electron microscopy (SEM) and atomic force microscopy (AFM). The analytical performances of the nanostructured-glasses were compared regarding biosensing of Hsp70, an ATP-driven molecular chaperone. In this work, the human heat-shock protein (Hsp70), was chosen as a model biomarker of body stress disorders for microwave spectroscopic investigations. It was found that microwave screening at 4 GHz allowed for the first time the detection of 12 ng/µL/cm2 of Hsp70.
