پژوهان (Jan 2020)
Pain Relief and Kaempferol: Activation of Transient Receptors Potential Vanilloid Type 1 in Male Rats
Abstract
Background and Objective: Pain an unpleasant sensory and emotional experience associated with actual or potential tissue damage, or described in terms of such damage. Kaempferol is one of the most important herbal active constituent with antinociceptive effects. The aim of this study was to evaluate the effects of intracerebroventricular injection of kaempferol and its interaction with the transient receptor potential cation channel subfamily V member 1 (TRPV1) on pain in male rats. Materials and Methods: In this experimental study, sixty male rats (200-250 g) were divided to the following groups: control (saline), Dimethyl sulfoxide (DMSO), morphine, kaempferol at dosages of 0.5, 1, and 1.5 mg/rat, capsaicin, capsazepine, capsaicin plus kaempferol (1.5 mg/rat), capsazepine plus kaempferol (1.5 mg/rat). After cannula implantation in cerebroventricular area, the rats received target components and then evaluated by pain assessment tests (abdominal writhing, tail-flick, and formalin tests). Data were analyzed by one-way ANOVA followed by Tukey’s post-test and P<0.05 was as a significant difference. Results: The results showed that administration of kaempferol had significant analgesic effects in comparison to the control/DMSO groups on the tail-flick, abdominal writhing, and formalin tests (P<0.05). Co-administration of capsaicin and kaempferol (1.5 mg/ rat) had significant analgesic effects compared to the control/DMSO groups although, not a synergist. Moreover, co-administration of capsazepine and kaempferol (1.5 mg/ rat) mostly decreased antinociceptive effects of kaempferol. Conclusion: The kaempferol probably has both acute and inflammatory antinociceptive effects and exert this activity at least in part by activating TRPV1 receptors.
