Development and Application of Real-Time PCR-Based Screening for Identification of Omicron SARS-CoV-2 Variant Sublineages

Anna Esman; Dmitry Dubodelov; Kamil Khafizov; Ivan Kotov; German Roev; Anna Golubeva; Gasan Gasanov; Marina Korabelnikova; Askar Turashev; Evgeniy Cherkashin; Konstantin Mironov; Anna Cherkashina; Vasily Akimkin

doi:10.3390/genes14061218

Genes (Jun 2023)

Development and Application of Real-Time PCR-Based Screening for Identification of Omicron SARS-CoV-2 Variant Sublineages

Anna Esman,
Dmitry Dubodelov,
Kamil Khafizov,
Ivan Kotov,
German Roev,
Anna Golubeva,
Gasan Gasanov,
Marina Korabelnikova,
Askar Turashev,
Evgeniy Cherkashin,
Konstantin Mironov,
Anna Cherkashina,
Vasily Akimkin

Affiliations

Anna Esman: Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia
Dmitry Dubodelov: Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia
Kamil Khafizov: Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia
Ivan Kotov: Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia
German Roev: Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia
Anna Golubeva: Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia
Gasan Gasanov: Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia
Marina Korabelnikova: Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia
Askar Turashev: Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia
Evgeniy Cherkashin: Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia
Konstantin Mironov: Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia
Anna Cherkashina: Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia
Vasily Akimkin: Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia

DOI: https://doi.org/10.3390/genes14061218
Journal volume & issue: Vol. 14, no. 6
p. 1218

Abstract

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The Omicron strain is currently the main dominant variant of SARS-CoV-2, with a large number of sublineages. In this article, we present our experience in tracing it in Russia using molecular diagnostic methods. For this purpose, different approaches were used; for example, we developed multiprimer panels for RT-PCR and Sanger and NGS sequencing methods. For the centralized collection and analysis of samples, the VGARus database was developed, which currently includes more than 300,000 viral sequences.

Published in Genes

ISSN: 2073-4425 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://www.mdpi.com/journal/genes/

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