Development of targeted nanocapsules using pomegranate peel extract with enhanced bioavailability and anti-inflammatory activity for ulcerative colitis: In vitro studies

P. Monika; M.N. Chandraprabha; R. Hari Krishna; Vittal Maanya; C. Likhitha; N. Pooja

Hybrid Advances (Dec 2024)

Development of targeted nanocapsules using pomegranate peel extract with enhanced bioavailability and anti-inflammatory activity for ulcerative colitis: In vitro studies

P. Monika,
M.N. Chandraprabha,
R. Hari Krishna,
Vittal Maanya,
C. Likhitha,
N. Pooja

Affiliations

P. Monika: Department of Biotechnology, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India; Corresponding author.
M.N. Chandraprabha: Department of Biotechnology, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India; Centre for Bio and Energy Materials Innovation, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India; Corresponding author. Department of Biotechnology, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India.
R. Hari Krishna: Centre for Bio and Energy Materials Innovation, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India; Department of Chemistry, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India; Corresponding author. Centre for Bio and Energy Materials Innovation, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India.
Vittal Maanya: Department of Biotechnology, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India
C. Likhitha: Department of Biotechnology, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India
N. Pooja: Department of Biotechnology, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India

Journal volume & issue: Vol. 7
p. 100334

Abstract

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The study aimed to establish an accurate drug delivery system with a tailored mechanism for the efficient management of Ulcerative Colitis (UC). Using the solvent evaporation technique, nanoencapsulation of pomegranate peel extract (PPE) in biodegradable enteric polymer methacrylic acid-ethyl acrylate copolymer (Eudragit L-100) was prepared. In vitro drug release investigations indicated a greater percentage of drug released at 6.8 pH in contrast to 7.4 pH (96.98 ± 0.80 % vs. 93.97 ± 0.711 %), indicating its applicability in the region of the human intestine. The mechanism of drug release from the nanoformulations (NFR) exhibited a process controlled by Fickian diffusion. The results suggest efficient encapsulation of PPE within Eudragit L100 and may be used for targeted drug release in the intestinal region. Anti-inflammatory studies on the HIEC-6 cell line showed that tumour necrosis factor-α (TNF-α) expression was significantly (p < 0.001) decreased in all NFRs. Additionally, NFRs exhibited antioxidant activity and enhanced in vitro bioavailability (in intestinal pH compared to gastric pH).

Published in Hybrid Advances

ISSN: 2773-207X (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology
Website: https://www.sciencedirect.com/journal/hybrid-advances

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