E3S Web of Conferences (Jan 2020)

Analysis of the species composition of microorganisms in dogs with otomycosis

  • Deneva Maria,
  • Orobets Vladimir,
  • Ozheredova Nadezhda,
  • Svetlakova Elena,
  • Grudeva Elena

DOI
https://doi.org/10.1051/e3sconf/202017503007
Journal volume & issue
Vol. 175
p. 03007

Abstract

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Otitis in dogs is a common inflammatory disease that is often complicated by secondary bacterial or yeast infections. In patients with chronic otitis externa, the disease is often caused by various combinations of factors. The object of the study was 237 sick dogs with clinical signs of otitis externa, of various age, sex and breed and a fixed population. Samples of the separated external auditory canal of sick dogs were used as material for mycological and bacteriological research. Material was sown on the surface of elective media. Microorganisms were isolated both in monoculture and bacterial-fungal associations. No microorganisms were detected in 14.7% of the samples. Yeast-like fungi - in 19.4% of the samples, bacteria - in 21.5%/ In the etiology of otitis associations of Malassezia and bacteria were established in 44.3% of the samples. As for species composition of fungi of the genus Malassezia, M. pachydermatis accounted for 72.4% and M. furfur - in 27.6% of samples. When analyzing the generic and species diversity of microorganisms, the most frequently recorded were coccal forms (87,0%), less frequently - enterobacteria, non-fermenting bacteria, streptococci, enterococci and corynebacteria. The results obtained can be used to develop effective tools of treatment for dogs with otitis externa.