Qualitative and Quantitative Determination of Decoquinate in Chicken Tissues by Gas Chromatography Tandem Mass Spectrometry
Shuyu Liu,
Yayun Tang,
Yang Lu,
Yawen Guo,
Kaizhou Xie,
Fanxun Guan,
Pengfei Gao,
Yali Zhu,
Yuhao Dong,
Tao Zhang,
Genxi Zhang,
Guojun Dai,
Xing Xie
Affiliations
Shuyu Liu
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Yayun Tang
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Yang Lu
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Yawen Guo
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Kaizhou Xie
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Fanxun Guan
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Pengfei Gao
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Yali Zhu
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Yuhao Dong
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
Tao Zhang
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Genxi Zhang
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Guojun Dai
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Xing Xie
Key Laboratory of Veterinary Biological Engineering and Technology, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Nanjing 210014, China
A novel precolumn derivatization–GC-MS/MS method was developed for the determination of decoquinate residues in chicken tissues (muscle, liver, and kidney). The samples were extracted and purified by liquid–liquid extraction combined with solid-phase extraction and derivatized with acetic anhydride and pyridine. The recovery rates for decoquinate were 77.38~89.65%, and the intra-day and inter-day RSDs were 1.63~5.74% and 2.27~8.06%, respectively. The technique parameters meet the necessities for veterinary drug residue detection in China, the US, and the EU. Finally, the method was applied to analyze tissues of 60 chickens bought from a neighborhood supermarket, and solely one sample of chicken muscle contained 15.6 μg/kg decoquinate residue.
