Application of M1 macrophage as a live vector in delivering nanoparticles for in vivo photothermal treatment

Nu-Ri Im; Taeseok Daniel Yang; Kwanjun Park; Jang-Hoon Lee; Jonghwan Lee; Yoon Hyuck Kim; Jae-Seung Lee; Byoungjae Kim; Kwang-Yoon Jung; Youngwoon Choi; Seung-Kuk Baek

Journal of Advanced Research (Jul 2021)

Application of M1 macrophage as a live vector in delivering nanoparticles for in vivo photothermal treatment

Nu-Ri Im,
Taeseok Daniel Yang,
Kwanjun Park,
Jang-Hoon Lee,
Jonghwan Lee,
Yoon Hyuck Kim,
Jae-Seung Lee,
Byoungjae Kim,
Kwang-Yoon Jung,
Youngwoon Choi,
Seung-Kuk Baek

Affiliations

Nu-Ri Im: Department of Otolaryngology-Head and Neck Surgery, Korea University, Seoul 02841, South Korea
Taeseok Daniel Yang: Department of Biomedical Engineering, Brown University, Providence, RI 02912, USA; School of Biomedical Engineering, Korea University, Seoul 02841, South Korea
Kwanjun Park: Department of Bioengineering, Korea University, Seoul 02841, South Korea
Jang-Hoon Lee: Department of Biomedical Engineering, Brown University, Providence, RI 02912, USA
Jonghwan Lee: Department of Biomedical Engineering, Brown University, Providence, RI 02912, USA
Yoon Hyuck Kim: Department of Materials Science and Engineering, Korea University, Seoul 02841, South Korea
Jae-Seung Lee: Department of Materials Science and Engineering, Korea University, Seoul 02841, South Korea
Byoungjae Kim: Department of Otolaryngology-Head and Neck Surgery, Korea University, Seoul 02841, South Korea; Department of Neuroscience Research Institute, Korea University, Seoul 02841, South Korea
Kwang-Yoon Jung: Department of Otolaryngology-Head and Neck Surgery, Korea University, Seoul 02841, South Korea
Youngwoon Choi: School of Biomedical Engineering, Korea University, Seoul 02841, South Korea; Department of Bioengineering, Korea University, Seoul 02841, South Korea; Interdisciplinary Program in Precision Public Health, Korea University, Seoul 02841, South Korea
Seung-Kuk Baek: Department of Otolaryngology-Head and Neck Surgery, Korea University, Seoul 02841, South Korea

Journal volume & issue: Vol. 31
pp. 155 – 163

Abstract

Read online

Introduction: To enhance photothermal treatment (PTT) efficiency, a delivery method that uses cell vector for nanoparticles (NPs) delivery has drawn attention and studied widely in recent years. Objectives: In this study, we demonstrated the feasibility of M1 activated macrophage as a live vector for delivering NPs and investigated the effect of NPs loaded M1 stimulated by Lipopolysaccharide on PTT efficiency in vivo. Methods: M1 was used as a live vector for delivering NPs and further to investigate the effect of NPs loaded M1 on PTT efficiency. Non-activated macrophage (MФ) was stimulated by lipopolysaccharide (LPS) into M1 and assessed for tumor cell phagocytic capacity towards NPs Results: We found M1 exhibited a 20-fold higher uptake capacity of NPs per cell volume and 2.9-fold more active infiltration into the tumor site, compared with non-activated macrophage MФ. We injected M1 cells peritumorally and observed that these cells penetrated into the tumor mass within 12 h. Then, we conducted PTT using irradiation of a near-infrared laser for 1 min at 1 W/cm2. As a result, we confirmed that using M1 as an active live vector led to a more rapid reduction in tumor size within 1 day indicating that the efficacy of PTT with NPs-loaded M1 is higher than that with NPs-loaded MФ. Conclusion: Our study demonstrated the potential role of M1 as a live vector for enhancing the feasibility of PTT in cancer treatment.

Published in Journal of Advanced Research

ISSN: 2090-1232 (Print); 2090-1224 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Science (General)
Website: http://www.journals.elsevier.com/journal-of-advanced-research/

About the journal

Abstract

Keywords