EFFECT OF SEROTONIN ON IMMUNE COMPETENT CELLS OF BIOMODELS UNDER THE CONDITIONS OF VACCINATION AGAINST PLAGUE AND TULAREMIA

Abstract

Aim. Comparative evaluation of the effect of exogenic serotonin on the development of apoptosis and proliferative activity of immune system cells of biomodels in vivo and in vitro in the dynamic of immunity forming against plague and tularemia. Materials and methods. Analysis of relative content of immune competent cell DNA of unlinear and BALB/c mice was carried out after staining of the samples with mithramycin and ethidium bromide by flow cytometry. Results. Administration of serotonin into biomodels before immunization with vaccine strains of Yersinia pestis and Francisella tularensis was established to increase in vivo proliferative activity of immune system cells, without a significant effect on their death by apoptosis. Serotonin inhibited in vitro the development of apoptosis of mice blood leukocytes in response to both the vaccine Y. pestis EV strain and tularin. Conclusion. Biogenic amine serotonin shows equivalent modulating effect on both anti-plague and anti-tularemia immune response in vivo and in vitro, without disrupting immune system homeostasis.

Keywords

• yersinia pestis
• francisella tularensis
• yersinia pestis
• francisella tularensis
• serotonin
• immune competent cells
• apoptosis
• proliferation