Regulation of MRI contrast and cellular retention time by cellular interfacial distribution of Gd agents: Implications for stem cell tracking

Yanhui Zhang; Binbin Li; Bo Tan; Hailu Zhang; Zongwu Deng

Magnetic Resonance Letters (Feb 2022)

Regulation of MRI contrast and cellular retention time by cellular interfacial distribution of Gd agents: Implications for stem cell tracking

Yanhui Zhang,
Binbin Li,
Bo Tan,
Hailu Zhang,
Zongwu Deng

Affiliations

Yanhui Zhang: School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei, 230026, China; CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou, 215123, China
Binbin Li: CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou, 215123, China
Bo Tan: CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou, 215123, China; Corresponding authors:
Hailu Zhang: CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou, 215123, China; Corresponding authors:
Zongwu Deng: School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei, 230026, China; CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou, 215123, China; Corresponding authors:

Journal volume & issue: Vol. 2, no. 1
pp. 48 – 55

Abstract

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Human mesenchymal stem cells (hMSCs) were labeled with Dotarem or (Gd-DOTA)2- EM7 (EM7Gd2) via electroporation (EP). Cellular transmission electron microscopy (TEM) reveals free distribution of Gd agents and formation of EM7Gd2 clusters in the cytosol. Cellular magnetic resonance imaging (MRI) reveals that the free Gd agents induce MRI signal enhancement effect due to its fast exocytosis and subsequent interaction with intercellular water molecules. The EM7Gd2 clusters exhibits a longer intracellular retention time and induce a persistent MRI signal reduction effect. The cellular MRI results are interpreted by taking into account both T1 and T2 relaxation rates and their correlation with cellular binding structures of Dotarem and EM7Gd2.

Published in Magnetic Resonance Letters

ISSN: 2097-0048 (Print); 2772-5162 (Online)
Publisher: KeAi Communications Co. Ltd.
Country of publisher: China
LCC subjects: Science: Chemistry: Physical and theoretical chemistry; Science: Chemistry: Analytical chemistry
Website: https://www.keaipublishing.com/en/journals/magnetic-resonance-letters/

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