Anti-E. coli Immunoglobulin Yolk (IgY): Reduction of pathogen receptors and inflammation factors could be caused by decrease in E. coli load
Abdellatif Bouazzaoui,
Neda M. Bogari,
Faisal A. Al-Allaf,
Samar N. Ekram,
Mohammad Athar,
Anas Dannoun,
Thomas Schubert,
Shahzad N. Syed,
Abdel-Rahman Youssef,
Mashael Alqahtani,
Ahmed A.H. Abdellatif
Affiliations
Abdellatif Bouazzaoui
Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, 21955, Makkah, Saudi Arabia; Science and Technology Unit, Umm Al-Qura University, 21955, Makkah, Saudi Arabia; Corresponding author. Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, 21955 Makkah, Saudi Arabia.
Neda M. Bogari
Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, 21955, Makkah, Saudi Arabia
Faisal A. Al-Allaf
Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, 21955, Makkah, Saudi Arabia
Samar N. Ekram
Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, 21955, Makkah, Saudi Arabia
Mohammad Athar
Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, 21955, Makkah, Saudi Arabia; Science and Technology Unit, Umm Al-Qura University, 21955, Makkah, Saudi Arabia
Anas Dannoun
Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, 21955, Makkah, Saudi Arabia
Thomas Schubert
Institut für angewandte Pathologie Speyer, Alter Postweg 1, 67346, Speyer, Germany
Shahzad N. Syed
Institute of Biochemistry I, Faculty of Medicine, Goethe-University Frankfurt, 60590 Frankfurt, Germany
Abdel-Rahman Youssef
Department of Basic and Clinical Oral Sciences, Faculty of Dentistry, Umm Al-Qura University, 21955, Makkah, Saudi Arabia
Mashael Alqahtani
Department of Basic and Clinical Oral Sciences, Faculty of Dentistry, Umm Al-Qura University, 21955, Makkah, Saudi Arabia
Ahmed A.H. Abdellatif
Department of Pharmaceutics, College of Pharmacy, Qassim University, 51452, Qassim, Saudi Arabia; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Al-Azhar University, 71524, Assiut, Egypt
Graft versus host disease (GVHD) remains the major cause of morbidity and mortality after allogeneic stem cell transplantation, especially for intestinal GVHD, as steroid resistant GVHD results in high mortality. For this reason, new treatments of GVHD are needed. One approach is the reduction of pathogenic bacteria using anti-E. coli Immunoglobulin Yolk (IgY). In a haploidentical murine model, B6D2F1 mice conditioned with total body irradiation (TBI), received bone marrow cells (BM) and splenocytes (SC) from either syngeneic (Syn = B6D2F1) or allogeneic (Allo = C57BL/6) donors. Following this, animals received from day −2 until day +28 chow contained IgY or control chow. Thereafter the incidence and severity of aGVHD, the cytokines, chemokines, IDO1 and different pathogen-recognition receptors (PRR) were analyzed and compared to control animals (received chow without IgY). We found that animals receiving chow with IgY antibody showed reduced GVHD severity compared to control animals. On day28 after alloBMT, IDO, NOD2, TLR2, TLR4 and the inflammatory chemokine CCL3, were reduced in the colon and correlated with a significant decrease in E. coli bacteria. In summary chow containing chicken antibodies (IgY) improved GVHD via decrease in bacterial load of E coli conducting to reduction of pathogen receptors (NOD2, TLR2 and 4), IDO, chemokines and cytokines.
