Frontiers in Cellular and Infection Microbiology (Apr 2021)
Dynamic Changes in Microbiome Composition Following Mare’s Milk Intake for Prevention of Collateral Antibiotic Effect
- Almagul Kushugulova,
- Almagul Kushugulova,
- Almagul Kushugulova,
- Ulrike Löber,
- Ulrike Löber,
- Ulrike Löber,
- Ulrike Löber,
- Saniya Akpanova,
- Kairat Rysbekov,
- Samat Kozhakhmetov,
- Samat Kozhakhmetov,
- Samat Kozhakhmetov,
- Zhanagul Khassenbekova,
- Morgan Essex,
- Morgan Essex,
- Morgan Essex,
- Ayaulym Nurgozhina,
- Ayaulym Nurgozhina,
- Madiyar Nurgaziyev,
- Madiyar Nurgaziyev,
- Dmitriy Babenko,
- Lajos Markó,
- Lajos Markó,
- Lajos Markó,
- Lajos Markó,
- Sofia K. Forslund,
- Sofia K. Forslund,
- Sofia K. Forslund,
- Sofia K. Forslund,
- Sofia K. Forslund
Affiliations
- Almagul Kushugulova
- Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan
- Almagul Kushugulova
- Kazakhstan Society of Human Microbiome Researchers, Nur-Sultan, Kazakhstan
- Almagul Kushugulova
- SaumalBioTech, Nur-Sultan, Kazakhstan
- Ulrike Löber
- Experimental and Clinical Research Center, a Cooperation of Charité-Universitätsmedizin Berlin and Max Delbrück Center for Molecular Medicine, Berlin, Germany
- Ulrike Löber
- Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany
- Ulrike Löber
- Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany
- Ulrike Löber
- DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany
- Saniya Akpanova
- Department of Pediatric Diseases with Courses in Cardio-Rheumatology and Gastroenterology, Nur-sultan (Astana) Medical University, Nur-Sultan, Kazakhstan
- Kairat Rysbekov
- Department of Pediatric Diseases with Courses in Cardio-Rheumatology and Gastroenterology, Nur-sultan (Astana) Medical University, Nur-Sultan, Kazakhstan
- Samat Kozhakhmetov
- Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan
- Samat Kozhakhmetov
- Kazakhstan Society of Human Microbiome Researchers, Nur-Sultan, Kazakhstan
- Samat Kozhakhmetov
- SaumalBioTech, Nur-Sultan, Kazakhstan
- Zhanagul Khassenbekova
- Kazakhstan Society of Human Microbiome Researchers, Nur-Sultan, Kazakhstan
- Morgan Essex
- Experimental and Clinical Research Center, a Cooperation of Charité-Universitätsmedizin Berlin and Max Delbrück Center for Molecular Medicine, Berlin, Germany
- Morgan Essex
- Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany
- Morgan Essex
- Laboratory of Innate Immunity, Department of Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin, Berlin, Germany
- Ayaulym Nurgozhina
- Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan
- Ayaulym Nurgozhina
- Kazakhstan Society of Human Microbiome Researchers, Nur-Sultan, Kazakhstan
- Madiyar Nurgaziyev
- Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan
- Madiyar Nurgaziyev
- Kazakhstan Society of Human Microbiome Researchers, Nur-Sultan, Kazakhstan
- Dmitriy Babenko
- 0Research Center Karaganda Medical University, Karagandy, Kazakhstan
- Lajos Markó
- Experimental and Clinical Research Center, a Cooperation of Charité-Universitätsmedizin Berlin and Max Delbrück Center for Molecular Medicine, Berlin, Germany
- Lajos Markó
- Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany
- Lajos Markó
- Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany
- Lajos Markó
- DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany
- Sofia K. Forslund
- Experimental and Clinical Research Center, a Cooperation of Charité-Universitätsmedizin Berlin and Max Delbrück Center for Molecular Medicine, Berlin, Germany
- Sofia K. Forslund
- Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany
- Sofia K. Forslund
- Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany
- Sofia K. Forslund
- DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany
- Sofia K. Forslund
- 1Structural and Computational Biology Unit, The European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
- DOI
- https://doi.org/10.3389/fcimb.2021.622735
- Journal volume & issue
-
Vol. 11
Abstract
IntroductionProbiotics and prebiotics are widely used for recovery of the human gut microbiome after antibiotic treatment. High antibiotic usage is especially common in children with developing microbiome. We hypothesized that dry Mare’s milk, which is rich in biologically active substances without containing live bacteria, could be used as a prebiotic in promoting microbial diversity following antibiotic treatment in children. The present pilot study aims to determine the impacts of dry Mare’s milk on the diversity of gut bacterial communities when administered during antibiotic treatment and throughout the subsequent recovery phase.MethodsSix children aged 4 to 5 years and diagnosed with bilateral bronchopneumonia were prescribed cephalosporin antibiotics. During the 60 days of the study, three children consumed dry Mare’s milk whereas the other three did not. Fecal samples were collected daily during antibiotic therapy and every 5 days after antibiotic therapy. Total DNA was isolated and taxonomic composition of gut microbiota was analyzed by 16S rRNA amplicon sequencing. To assess the immune status of the gut, stool samples were analyzed by bead-based multiplex assays.ResultsMare’s milk treatment seems to prevent the bloom of Mollicutes, while preventing the loss of Coriobacteriales. Immunological analysis of the stool reveals an effect of Mare’s milk on local immune parameters under the present conditions.
Keywords
