Conversion of Sweet Whey to Bioethanol: A Bioremediation Alternative for Dairy Industry
Laura Conde-Báez,
Cuauhtémoc F. Pineda-Muñoz,
Carolina Conde-Mejía,
Elizabeth Mas-Hernández,
Antioco López-Molina
Affiliations
Laura Conde-Báez
Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas, s/n, Ciudad Universitaria, Chilpancingo 39086, Guerrero, Mexico
Cuauhtémoc F. Pineda-Muñoz
Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas, s/n, Ciudad Universitaria, Chilpancingo 39086, Guerrero, Mexico
Carolina Conde-Mejía
División Académica Multidisciplinaria de Jalpa de Méndez, Universidad Juárez Autónoma de Tabasco, Carretera Estatal Libre Villahermosa-Comalcalco Km 27 s/n, Ranchería Ribera Alta, Jalpa de Méndez 86205, Tabasco, Mexico
Elizabeth Mas-Hernández
Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Col. de las Campanas, Santiago de Querétaro 76010, Querétaro, Mexico
Antioco López-Molina
División Académica Multidisciplinaria de Jalpa de Méndez, Universidad Juárez Autónoma de Tabasco, Carretera Estatal Libre Villahermosa-Comalcalco Km 27 s/n, Ranchería Ribera Alta, Jalpa de Méndez 86205, Tabasco, Mexico
In many countries, whey from the dairy industry is an abundant waste that generates an important environmental impact. Alternative processes to use the whey and minimize the environmental impact are needed. This work considered six formulations with different ammonium sulfate and L-phenylalanine (L-Phe) concentrations to produce bioethanol in sweet whey fermentation by Kluyveromyces marxianus. The results showed a maximum bioethanol concentration equal to 25.13 ± 0.37 g L−1 (p −1 of L-Phe and 1.350 g L−1 of ammonium sulfate (96 h). For these conditions, the chemical oxygen demand removal percentage (CODR%) was 67%. The maximum CODR% obtained was 97.5% for formulation F3 (1 g L−1 of L-Phe) at 96 h; however, a significant decrease in bioethanol concentration (14.33 ± 2.58 g L−1) was observed. On the other hand, for formulation, F3, at 48 h of fermentation time, a bioethanol concentration of 23.71 ± 1.26 g L−1 was observed, with 76.5% CODR%. Based on these results, we suggest that the best conditions to obtain a significant bioethanol concentration and CODR% value are those used on the configuration F3 at 48 h.