Stem Cell Research (Dec 2017)

Generation and characterization of human iPSC lines SANi001-A and SANi002-A from mobilized peripheral blood derived megakaryoblasts

  • Marten Hansen,
  • Eszter Varga,
  • Tatjana Wüst,
  • Clemens Mellink,
  • Anne-Marie van der Kevie-Kersemaekers,
  • Marieke von Lindern,
  • Emile van den Akker

DOI
https://doi.org/10.1016/j.scr.2017.10.002
Journal volume & issue
Vol. 25, no. C
pp. 42 – 45

Abstract

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Mobilized peripheral blood (MPB) CD34+ cells were differentiated to CD34+/CD41+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population which was reprogrammed with a hOKSM self-silencing polycistronic lentiviral vector. Resulting iPSC showed normal karyotype and expression of pluripotency associated markers and in vitro spontaneous differentiation towards the 3 germ layers confirmed pluripotency of iPSC lines. Besides normal iPSC applications, these lines can be used as a control line for other megakaryoid origin iPSC and could be applied for epigenetic based research.