Antibiotic and disinfectant resistance of Salmonella enterica Typhimurium

Xueping HE; Xuebin XU; Aiping LIU; Li HE; Yong YANG; Xiaolin AO; Kang ZHOU; Shujuan CHEN; Likou ZOU

doi:10.13590/j.cjfh.2020.04.002

Zhongguo shipin weisheng zazhi (Jul 2020)

Antibiotic and disinfectant resistance of Salmonella enterica Typhimurium

Xueping HE,
Xuebin XU,
Aiping LIU,
Li HE,
Yong YANG,
Xiaolin AO,
Kang ZHOU,
Shujuan CHEN,
Likou ZOU

Affiliations

Xueping HE: College of Food Science, Sichuan Agricultural University, Sichuan Ya’an 625014, China
Xuebin XU: Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China
Aiping LIU: College of Food Science, Sichuan Agricultural University, Sichuan Ya’an 625014, China
Li HE: College of Food Science, Sichuan Agricultural University, Sichuan Ya’an 625014, China
Yong YANG: College of Food Science, Sichuan Agricultural University, Sichuan Ya’an 625014, China
Xiaolin AO: College of Food Science, Sichuan Agricultural University, Sichuan Ya’an 625014, China
Kang ZHOU: College of Food Science, Sichuan Agricultural University, Sichuan Ya’an 625014, China
Shujuan CHEN: College of Food Science, Sichuan Agricultural University, Sichuan Ya’an 625014, China
Likou ZOU: College of Resources, Sichuan Agricultural University, Sichuan Chengdu 611130, China

DOI: https://doi.org/10.13590/j.cjfh.2020.04.002
Journal volume & issue: Vol. 32, no. 04
pp. 356 – 363

Abstract

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Objective The aims of this study were to investigate the antibiotic resistance, disinfectant resistance and their associations in 302 Salmonella enterica Typhimurium (S. Typhimurium) isolates from retail meats, food animals and humans. Methods Antibiotic susceptibility testing was performed according to the Kirby-Bauer disk diffusion method. The minimal inhibitory concentrations (MICs) of disinfectants were determined by broth microdilution method, and disinfectant resistance genes were detected by polymerase chain reaction (PCR) amplification. Results Antibiotic susceptibility testing demonstrated that 96.03% (290/302) of S. Typhimurium isolates were resistant to at least one antibiotic and 79.80% (241/302) were multidrug resistant (MDR). Tested isolates showed the highest resistance to streptomycin (78.81%, 238/302), followed by compound sulphonamides (78.15%, 236/302), tetracycline (75.50%, 228/302) and ampicillin (71.85%, 217/302). All isolates were sensitive to cefepime and imipenem. The resistance of S. Typhimurium from food to compound sulphonamides, tetracycline, ampicillin, nalidixic acid, and gentamicin were significantly higher than those from human and animal origin (P1 024 mg/L, respectively. In addition, the frequency of resistance to PVP-I, BC and TCS was observed significantly higher in food and animal isolates than human isolates (P<0.05). The qacEΔ1, sugE (p) and qacE disinfectant resistance genes were detected in 56.95% (172/302), 20.53% (62/302) and 2.65% (8/302) of all the isolates, respectively. Notably, qacEΔ1 gene was significantly associated with β-lactam, aminoglycoside, tetracycline, sulfonamide, quinolone and chloramphenicol resistance (P<0.01). Conclusion It was indicated that antibiotic and disinfectant resistance were common among S. Typhimurium isolates and the use of disinfectants or antibiotics may contribute to co-selecting isolates with acquired resistance to other antimicrobials, which could pose significant threats to food safety and public health.

Published in Zhongguo shipin weisheng zazhi

ISSN: 1004-8456 (Print)
Publisher: The Editorial Office of Chinese Journal of Food Hygiene
Country of publisher: China
LCC subjects: Technology: Chemical technology: Food processing and manufacture; Technology: Home economics: Nutrition. Foods and food supply
Website: http://www.zgspws.com/zgspwszzen/home

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