New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA

Andres Pascual; Katia Jaton; Béatrice Ninet; Jacques Bille; Gilbert Greub

doi:10.1155/2010/317512

International Journal of Microbiology (Jan 2010)

New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA

Andres Pascual,
Katia Jaton,
Béatrice Ninet,
Jacques Bille,
Gilbert Greub

Affiliations

Andres Pascual: Infectious Diseases Service, Department of Internal Medicine, University Hospital Center and University of Lausanne, 1011 Lausanne, Switzerland
Katia Jaton: Institute of Microbiology, University Hospital Center and University of Lausanne, 1011 Lausanne, Switzerland
Béatrice Ninet: Laboratory of Microbiology, University Hospital of Geneva, Geneva, Switzerland
Jacques Bille: Infectious Diseases Service, Department of Internal Medicine, University Hospital Center and University of Lausanne, 1011 Lausanne, Switzerland
Gilbert Greub: Infectious Diseases Service, Department of Internal Medicine, University Hospital Center and University of Lausanne, 1011 Lausanne, Switzerland

DOI: https://doi.org/10.1155/2010/317512
Journal volume & issue: Vol. 2010

Abstract

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Mycoplasma hominis is a fastidious micro-organism causing genital and extragenital infections. We developed a specific real-time PCR that exhibits high sensitivity and low intrarun and interrun variabilities. When applied to clinical samples, this quantitative PCR allowed to confirm the role of M. hominis in three patients with severe extragenital infections.

Published in International Journal of Microbiology

ISSN: 1687-918X (Print); 1687-9198 (Online)
Publisher: Hindawi Limited
Country of publisher: United Kingdom
LCC subjects: Science: Microbiology
Website: https://www.hindawi.com/journals/ijmicro/

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