Фармацевтичний журнал (Feb 2022)
Phytochemical characteristics and the study of acute toxicity of extracts from fruit Рrunus domestica
Abstract
The increased demand for modern phytopreparations is dictated by their wide application in the complex treatment of various diseases, the presence of low toxicity and the pharmacoeconomic component. Phytochemical analysis of medicinal plants reveals not only potential therapeutic effects, but also assumes their toxicological properties. In this context, the Prunus domestica, widely spread on the territory of our country and well known for its medicinal properties and applications in folk medicine, has attracted our attention. The aim of the work was to investigate the phytochemical composition of extracts obtained from fruit Prunus domestica and to study their toxic properties. Phytochemical analysis of the extracts obtained from fruits Prunus domestica was carried out according to the original method of extracting biologically active compounds, using instrumental research methods using modern laboratory analytical equipment. Studies of the safety of four extracts from Prunus domestica (liquid EC-1, EC-2 and dry CEPC, CEV) with the calculation of median lethal doses were carried out on mice and rats with the single intragastric and intraperitoneal routes of administration according to the method of T. V. Pastushenko. The degree of toxicity was determined according to the classification of K. K. Sidorov. Phytochemical study of extracts from fruit Prunus domestica provided information on the presence in their chemical composition of flavonoids, the sum of phenolic compounds, anthocyanins, neutral sugars, amino acids, organic and hydroxycinnamic acids. A higher content of the sum of phenolic compounds in the aqueous (ЕС-1) and alcoholic extracts (ЕС-2) as well as the presence of neutral sugars as products of hydrolysis of homopolysaccharides (СEV extract) and heteropolysaccharides (CEPC extract). Experimental studies proved that EC-1, EC-2, CEPC and SEV extracts had low toxicity beyond 15 000 mg/kg when administered orally and 3 000 mg/kg when administered intraperitoneally. The LD50 of the studied extracts of mice and rats after intraperitoneal injection was determined, which was more than 3 000 mg/kg. The chemical composition of biologically active compounds of extracts from the fruit Prunus domestica, which provides promising directions for further study of their pharmacological properties, has been investigated. Analysis of experimental data on acute toxicity of the studied extracts showed that they belong to the IV class of toxicity “relatively harmless substances", according to K. K. Sidorov classification. The LD50 of extracts from the fruit Prunus domestica for oral administration could not be determined, and the LD50 of the studied extracts for mice and rats after intraperitoneal administration was more than 3 000 mg/kg.
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