Mode of action of antifouling compound albofungin in inhibiting barnacle larval settlement
Weiyi She,
Hao Wang,
Darwin Linardi,
Sin Yu Chik,
Yi Lan,
Feng Chen,
Aifang Cheng,
Pei-Yuan Qian
Affiliations
Weiyi She
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Nansha, Guangdong, China; Department of Ocean Science and Hong Kong Brach of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Hong Kong University of Science and Technology, Hong Kong, China; SZU-HKUST Joint PhD Program in Marine Environmental Science, Shenzhen University, Shenzhen 518060, China
Hao Wang
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Nansha, Guangdong, China; Department of Ocean Science and Hong Kong Brach of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Hong Kong University of Science and Technology, Hong Kong, China
Darwin Linardi
Chemical and Biological Engineering, Hong Kong University of Science and Technology, Hong Kong, China
Sin Yu Chik
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Nansha, Guangdong, China; Department of Ocean Science and Hong Kong Brach of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Hong Kong University of Science and Technology, Hong Kong, China
Yi Lan
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Nansha, Guangdong, China; Department of Ocean Science and Hong Kong Brach of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Hong Kong University of Science and Technology, Hong Kong, China
Feng Chen
Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China
Aifang Cheng
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Nansha, Guangdong, China; Department of Ocean Science and Hong Kong Brach of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Hong Kong University of Science and Technology, Hong Kong, China; Corresponding author
Pei-Yuan Qian
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Nansha, Guangdong, China; Department of Ocean Science and Hong Kong Brach of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Hong Kong University of Science and Technology, Hong Kong, China; Corresponding author
Summary: Marine biofouling causes huge economic losses to the marine industry every year. Albofungin is a potential antifoulant showing strong anti-macrofouling activities against larval settlement of major fouling organisms. In the present study, directed RNA-seq and proteomic analyses were used to investigate changes in the transcriptome and proteome of a major fouling barnacle Amphibalanus amphitrite cyprids in response to albofungin treatment. Results showed that albofungin treatment remarkably upregulated the metabolism of xenobiotics by the cytochrome P450 pathway to discharge the compound and downregulated energy metabolic processes. Intriguingly, immunostaining and whole-mount in situ hybridization (WISH) revealed the spatial expression patterns of selected differentially expressed genes (glutathione S-transferase [GST], nitric oxide synthase [NOS], and calmodulin [CaM]) distributed in the thorax and antennule of A. amphitrite. Our study provides new insights into the mechanism of albofungin in interrupting the larval settlement of A. amphitrite and suggests its potential application as an antifouling agent in marine environments.
