The Effects of Six Weeks Endurance Training on XBP-1 Protein in the Diabetic Male Wistar Rats Sciatica Tissues

Abstract

Objective: The aim of this study was to investigate the effect of endurance activity on XBP-1 protein content in sciatica tissue of diabetic male Wistar rats. Materials and Methods: The experimental design was a post-test design with control group. In this study, 32 male Wistar 10 weeks old rats with the mean weight of 253.16 (±10.16) grams were randomly divided into four groups: healthy control, healthy exercise, diabetic control and diabetic training group. An intraperitoneal injection of STZ (45 mg / kg) solution was used to induce diabetes after 12 hours fasting, 48 hours after STZ injection, the moderate-intensity endurance protocol was performed for 6 weeks. Twenty-four hours after the last training session, the mice were exposed and their sciatic nerve was extracted. The amount of XBP-1 protein was measured by ELISA method. For statistical analysis, SPSS software and two-way ANOVA with repeated measure and independent T-test were used. Results: Data analysis showed that endurance activity had an effect on the amount of XBP-1 protein and reduced the protein content, so that the difference was significant in the diabetic training group compared to the diabetic control group (P-value: 0.007). Conclusion: These findings indicated that moderate aerobic exercise has a significant effect on the amount of XBP-1 protein in healthy and diabetic rats.

Keywords

• Endoplasmic reticulum
• Diabetes
• Sciatic nerve
• XBP-1 protein